SDS-Page
(Purity: Sample purity data. For lot-specific data, please refer to the lot-specific datasheet.)
LbuCas13a recombinant protein
LbuCas13a, Active
Synonyms
LbuCas13a; Active; LbuCas13a recombinant protein
Host
E Coli
Form/Format
Recombinant protein stored in 50mM sodium phosphate, pH7.5, 300mM NaCl, 1mM DTT, 10% glycerol.
Tag
Tag-free
Species
Leptotrichia buccalis
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-Page
(Purity: Sample purity data. For lot-specific data, please refer to the lot-specific datasheet.)
SDS-Page
(Purity: Sample purity data. For lot-specific data, please refer to the lot-specific datasheet.)
Activity
(Specific Activity: Sample activity data. for lot-specific data, please refer to the lot-specific datasheet)
Activity
(Specific Activity: Sample activity data. for lot-specific data, please refer to the lot-specific datasheet)
Related Product Information for LbuCas13a recombinant protein
Recombinant tag-free Leptotrichia buccalis LbuCas13a was expressed in E Coli cells. The protein accession number is WP_015770004.
Cas13a is an RNA-guided RNA endonuclease that belongs to the class 2 type VI CRISPR-Cas system (1). Cas13a possesses both RNA recognition and cleavage activities and cleaves RNA through conserved basic residues within its two HEPN domains (2). Interestingly, activated Cas13a induces collateral cleavage of nearby non-targeted RNAs in a nonspecific manner (3). Engineered LbuCas13a shows stability and altered cleavage activity in vitro and possesses the capability of robust exon skipping in human cells (4).
Cas13a is an RNA-guided RNA endonuclease that belongs to the class 2 type VI CRISPR-Cas system (1). Cas13a possesses both RNA recognition and cleavage activities and cleaves RNA through conserved basic residues within its two HEPN domains (2). Interestingly, activated Cas13a induces collateral cleavage of nearby non-targeted RNAs in a nonspecific manner (3). Engineered LbuCas13a shows stability and altered cleavage activity in vitro and possesses the capability of robust exon skipping in human cells (4).
Product Categories/Family for LbuCas13a recombinant protein
References
1. East-Seletsky A, et al: Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 2016, 538:270-3. 2. Abudayyeh O, et al: C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 2016, 353:5573. 3. Patchsung M, et al: Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA. Nat Biomed Eng. 2020. https://doi.org/10.1038/s41551-020-00603-x. 4. Charles E, et al: Engineering improved Cas13 effectors for targeted post-transcriptional regulation of gene expression. bioRxiv. doi: https://doi.org/10.1101/2021.05.26.445687.
NCBI and Uniprot Product Information
Molecular Weight
132kDa
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Product Notes
The LbuCas13a (Catalog #AAA516770) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "LbuCas13a, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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