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9 results for "CRISPR Associated Proteins" - showing 1-9
Western Blot (WB)
(Western blot analysis of CRISPR-Cas9 SP expression in (1) 293T cell lysate transfected with CRISPR-Cas9; (2) 293T cell lysate; (3) 3T3 cell lysate; (4) PC12 cell lysate (MBS1750147).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-cas9 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for cas9 )
CRISPR-Cas9 SP, Monoclonal Antibody (Cat# AAA1750147)
Full Name
Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody
Reactivity
Streptococcus pygenes
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CRISPR-Cas9 expression in (1) 293T cell lysate transfected with CRISPR-Cas9; (2) 293T cell lysate; (3) 3T3 cell lysate; (4) PC12 cell lysate (MBS1750148).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-cas9 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for cas9 )
CRISPR-Cas9, Monoclonal Antibody (Cat# AAA1750148)
Full Name
Anti-CRISPR-Cas9 Rabbit Monoclonal Antibody
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
AapCas12b, Recombinant Protein (Cat# AAA516659)
Full Name
AapCas12b, Active
Purity
>75%
Pricing
$275/50 pmol | $355/100 pmol | $1,310/1000 pmol | $5,675/5x1000 pmol | $560/200 pmol
SDS-Page
(Purity: Sample purity data. For lot-specific data, please refer to the lot-specific datasheet.)
LbuCas13a, Recombinant Protein (Cat# AAA516770)
Full Name
LbuCas13a, Active
Pricing
$275/50 pmol | $355/100 pmol | $1,310/1000 pmol | $5,675/5x1000 pmol | $560/200 pmol
Activity
(Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet)
AsCas12a, Recombinant Protein (Cat# AAA516768)
Full Name
AsCas12a, Active
Pricing
$275/50 pmol | $355/100 pmol | $1,310/1000 pmol | $5,675/5x1000 pmol | $560/200 pmol
Activity
(Activity: Sample activity data. For lot-specific data, please refer to the specific datasheet.)
Cas13a (LwC2c2), Recombinant Protein (Cat# AAA516769)
Full Name
Cas13a (LwC2c2), Active
Pricing
$275/50 pmol | $355/100 pmol | $1,310/1000 pmol | $5,675/5x1000 pmol | $560/200 pmol
SDS-Page
(Recombinant dCas9 protein, His/AM Tag 7.5% SDS-PAGE gel stained with Coomassie Blue MW: 166 kDa Purity: > 80% )
dCas9, Recombinant Protein (Cat# AAA389127)
Full Name
Recombinant dCas9 protein, His/AM Tag
Pricing
$550/0.1 mg | $3,175/1 mg | $14,240/5x1 mg
SDS-Page
(Recombinant dCas9 protein, His / DYKDDDDK Tag 7.5% SDS-PAGE gel stained with Coomassie Blue. MW: 164.9 kDa Purity: > 85% )
dCas9, Recombinant Protein (Cat# AAA389128)
Full Name
Recombinant dCas9 protein, His/DYKDDDDK Tag
Pricing
$550/0.1 mg | $3,175/1 mg | $14,240/5x1 mg
SDS-Page
(Recombinant Cas9 protein gel 7.5% SDS-PAGE gel stained with Coomassie Blue. MW: 161.2 kDa Purity: > 85% )
Cas9, Recombinant Protein (Cat# AAA389126)
Full Name
Recombinant Cas9 protein
Pricing
$490/0.1 mg | $3,175/1 mg | $14,240/5x1 mg
