IKK alpha Recombinant Protein | IKKalpha recombinant protein

IKK alpha, Active

Cat. Num. AAA516735

• Gene Names: CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
• Applications: Western Blot
• Synonyms: IKK alpha; Active; CHUK; IKK1; IKBKA; TCF16; NFKBIKA; IKK-alpha; IKKalpha recombinant protein

• Host: Sf9 insect cells
• Form/Format: Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

• Applicable Applications for IKKalpha recombinant protein: Kinase Assay, Western Blot (WB)
• Tag: GST
• Species: Human
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Activity

(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)

SDS-Page

(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)

Related Product Information for IKKalpha recombinant protein

Full length recombinant human IKKalpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is BC092514.
IKKalpha is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein which is an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B protein triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex (1). IKKalpha is an essential regulator of NF-kappa-B-dependent gene expression through control of promoter-associated histone phosphorylation after cytokine exposure (2). IKKalpha is a critical component of the cytoplasmic transductional-transcriptional processor leading to induction of IFNalpha production. IKKalpha is also involved in the epidermis where it antagonizes mitogenic and angiogenic signals and represses tumor progression and metastases.

Product Categories/Family for IKKalpha recombinant protein

Protein Kinases; AKT/PKB Pathway; Apoptosis/Autophagy; Cancer; Inflammation; Neurobiology; NfkB Pathway; p38 Pathway; Ser/Thr Kinases; WNT Signaling

References

1. Anest, V.et.al: A nucleosomal function for I-kappa-B kinase-alpha in NF-kappa-B-dependent gene expression. Nature 423: 659-663, 2003.
2. Hoshino, K. et.al: I-kappa-B kinase-alpha is critical for interferon-alpha production induced by Toll-like receptors 7 and 9. Nature 440: 949-953, 2006.

NCBI and Uniprot Product Information

• NCBI GI #: 62531285
• NCBI GeneID: 1147
• Molecular Weight: 114.0 kDa
• NCBI Official Full Name: Homo sapiens conserved helix-loop-helix ubiquitous kinase, mRNA
• NCBI Official Synonym Full Names: component of inhibitor of nuclear factor kappa B kinase complex
• NCBI Official Symbol: CHUK
• NCBI Official Synonym Symbols: IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit alpha

NCBI Description

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]