AapCas12b recombinant protein
AapCas12b, Active
Synonyms
AapCas12b; Active; AapCas12b recombinant protein
Host
E Coli
Form/Format
Recombinant protein stored in 50mM sodium phosphate, pH7.5, 300mM NaCl, 1mM DTT, 10% glycerol.
Tag
Tag-free
Species
Alicyclobacillus acidiphilus
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Related Product Information for AapCas12b recombinant protein
Recombinant tag-free Alicyclobacillus acidiphilus AapCas12b was expressed in E Coli cells. The protein accession number is WP_067623834.
CRISPR (clustered regularly interspaced short palindromic repeat) and their CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria (1-2). This system has been redesigned to create an exemplary genome editing tool for application to RNA-based therapeutics development (3). AapCas12b belongs to the type V CRISPR effector, CRISPR-Cas12b/C2c1, and due to its thermostability, it can be used in a wide range of biomedical applications including loop-mediated isothermal amplification (LAMP), mammalian genome editing, and gene activation (4).
CRISPR (clustered regularly interspaced short palindromic repeat) and their CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria (1-2). This system has been redesigned to create an exemplary genome editing tool for application to RNA-based therapeutics development (3). AapCas12b belongs to the type V CRISPR effector, CRISPR-Cas12b/C2c1, and due to its thermostability, it can be used in a wide range of biomedical applications including loop-mediated isothermal amplification (LAMP), mammalian genome editing, and gene activation (4).
Product Categories/Family for AapCas12b recombinant protein
References
1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89. 2. Lucia C, et al: An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection method based on CRISPR-Cas12. bioRxiv. doi: https://doi.org/10.1101/2020.02.29.971127. 3. Teng F, et al: Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell discovery. 2018, 4(1):1-5.
NCBI and Uniprot Product Information
Molecular Weight
128kDa
