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(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
T4 gp32 Recombinant Protein | T4 recombinant protein
T4 gp32 Protein
Synonyms
T4 gp32; T4 gp32 Protein; ss-DNA binding protein (g32); SSB protein; Helix-destabilizing protein; T4 recombinant protein
Host
E Coli
Form/Format
Recombinant protein stored in 50mM sodium phosphate, pH7.5, 300mM NaCl, 20% glycerol, 1mM DTT.
Tag
HIS
Species
Escherichia virus T4
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for T4 recombinant protein
Recombinant full-length T4 gp32 Protein from Escherichia virus T4 was expressed in E Coli using N-terminal His tag. The gene accession number is J02513.
Bacteriophage T4 gene 32 protein (gp32) is a single-stranded DNA binding protein with key role in DNA replication, recombination, and repair. T4 gp32 is a zinc metalloprotein with three functional domains: the N-terminal basic domain, the core domain and the C-terminal acidic domain. The ssDNA binding site lies in the core domain. In vitro, T4 gp32 binds cooperatively to single stranded DNA and stimulates cell-free DNA synthesis directed by the T4-encoded DNA polymerase (1,2)
Bacteriophage T4 gene 32 protein (gp32) is a single-stranded DNA binding protein with key role in DNA replication, recombination, and repair. T4 gp32 is a zinc metalloprotein with three functional domains: the N-terminal basic domain, the core domain and the C-terminal acidic domain. The ssDNA binding site lies in the core domain. In vitro, T4 gp32 binds cooperatively to single stranded DNA and stimulates cell-free DNA synthesis directed by the T4-encoded DNA polymerase (1,2)
Product Categories/Family for T4 recombinant protein
References
1. Jordan, C.S. et al: Regulation of the bacteriophage T4 Dda helicase by Gp32 single-stranded DNA-binding protein. DNA Repair (Amst), 2015; 25: 41-53. 2. Pant, K. et al: The role of the C-domain of bacteriophage T4 gene 32 protein in ssDNA binding and dsDNA helix-destabilization: Kinetic, single-molecule, and cross-linking studies. PLoS One. 2018; 10:13(4):e0194357.
NCBI and Uniprot Product Information
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Product Notes
The T4 (Catalog #AAA517714) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "T4 gp32, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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