Western Blot (WB)
(Sample Data: Anti-p21CIP1 (p21/WAF1) detects human p21 by western blot. 10 ug of a HeLa whole cell lysate was loaded per lane and incubated with a 1:1,000 of Anti-p21CIP1 (p21/WAF1). A single band corresponding to human p21WAF1 is detected at ~21 kDa.)
Mouse anti-Human p21CIP1 (p21/WAF1) Monoclonal Antibody | anti-p21CIP1 antibody
Anti-p21CIP1 (p21/WAF1)
Reactivity
Human
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
Affinity chromatography
Synonyms
p21CIP1 (p21/WAF1); Monoclonal Antibody; Anti-p21CIP1 (p21/WAF1); anti-p21CIP1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse, IgG
Specificity
Recognizes the p21CIP1 (p21/WAF1) protein
Purity/Purification
Affinity chromatography
Form/Format
0.02M Potassium Phosphate, 0.5M Sodium Chloride, pH7.2, 0.01% (w/v) Sodium Azide
Applicable Applications for anti-p21CIP1 antibody
ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
Immunogen
Produced by repeated immunizations with recombinant human p21 fusion protein.
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
1 year at -20 degree C from date of shipment
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Anti-p21CIP1 (p21/WAF1) detects human p21 by western blot. 10 ug of a HeLa whole cell lysate was loaded per lane and incubated with a 1:1,000 of Anti-p21CIP1 (p21/WAF1). A single band corresponding to human p21WAF1 is detected at ~21 kDa.)
Western Blot (WB)
(Sample Data: Anti-p21CIP1 (p21/WAF1) detects human p21 by western blot. 10 ug of a HeLa whole cell lysate was loaded per lane and incubated with a 1:1,000 of Anti-p21CIP1 (p21/WAF1). A single band corresponding to human p21WAF1 is detected at ~21 kDa.)
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemistry of Anti-p21CIP1 (p21/WAF1) antibody 1:250. Tissue: human brain. Co-stained with YFP and Sox2 antibodies. Localization: p21 is nuclear and cytoplasmic. Staining: p21CIP1 as precipitated blue with Cy5, YFP as precipitated green with Cy2, and Sox2 as precipitated red with Cy3. z-stacks from confocal expressed as one composite focal plane.)
Immunohistochemistry (IHC)
(Sample Data: Immunohistochemistry of Anti-p21CIP1 (p21/WAF1) antibody 1:250. Tissue: human brain. Co-stained with YFP and Sox2 antibodies. Localization: p21 is nuclear and cytoplasmic. Staining: p21CIP1 as precipitated blue with Cy5, YFP as precipitated green with Cy2, and Sox2 as precipitated red with Cy3. z-stacks from confocal expressed as one composite focal plane.)
Related Product Information for anti-p21CIP1 antibody
CIP 1 (Cyclin-Dependent Kinase Inhibitor 1A) regulates cell cycle progression, terminal differentiation, and apoptosis (1). CIP1 is induced by p53 and is a potent inhibitor of cyclin-dependent kinase (CDK) activity. DNA damage leads to increased expression of CIP1 in cyclin E-containing complexes and to an associated decrease in cyclin-dependent kinase activity. CIP1 is a critical downstream effector in the p53-specific pathway of growth control in mammalian cells (2).
Product Categories/Family for anti-p21CIP1 antibody
References
1. el-Deiry, W S. et al: WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis. Cancer Res. 1994;54(5):1169-74.
2. Wagner, M. et al: Cyclin-dependent kinase-inhibitor 1 (CDKN1A) in the squamous epithelium of the oropharynx: possible implications of molecular biology and compartmentation. Anticancer Res. 2001; 21(1A):333-45.
2. Wagner, M. et al: Cyclin-dependent kinase-inhibitor 1 (CDKN1A) in the squamous epithelium of the oropharynx: possible implications of molecular biology and compartmentation. Anticancer Res. 2001; 21(1A):333-45.
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Product Notes
The p21CIP1 (Catalog #AAA515895) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-p21CIP1 (p21/WAF1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p21CIP1 (p21/WAF1) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the p21CIP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p21CIP1 (p21/WAF1), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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