Mouse Sorbitol Dehydrogenase ELISA Kit | SDH elisa kit

Mouse Sorbitol Dehydrogenase ELISA Kit

Cat. Num. AAA761115

• Gene Names: SORD; SORD1; HEL-S-95n
• Reactivity: Mouse
• Synonyms: Sorbitol Dehydrogenase; Mouse Sorbitol Dehydrogenase ELISA Kit; SDH; SDH elisa kit

• Reactivity: Mouse
• Sequence Length: 357

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 1.563-100ng/ml
• Sensitivity: 0.938ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for SDH elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse SDH antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse SDH antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse SDH amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse SDH can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 156627571
• NCBI GeneID: 6652
• NCBI Accession #: NP_003095.2
• NCBI GenBank Nucleotide #: NM_003104.5
• Molecular Weight: 10,812 Da
• NCBI Official Full Name: sorbitol dehydrogenase
• NCBI Official Synonym Full Names: sorbitol dehydrogenase
• NCBI Official Symbol: SORD
• NCBI Official Synonym Symbols: SORD1; HEL-S-95n
• NCBI Protein Information: sorbitol dehydrogenase
• UniProt Protein Name: Sorbitol dehydrogenase
• Protein Family: Serine 3-dehydrogenase
• UniProt Gene Name: SORD
• UniProt Entry Name: DHSO_HUMAN

NCBI Description

Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]

Uniprot Description

SORD: Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. Belongs to the zinc-containing alcohol dehydrogenase family.

Protein type: Oxidoreductase; Carbohydrate Metabolism - fructose and mannose; EC 1.1.1.14

Chromosomal Location of Human Ortholog: 15q15.3

Cellular Component: cytosol; extracellular space; membrane; mitochondrial membrane

Molecular Function: carbohydrate binding; D-xylulose reductase activity; identical protein binding; L-iditol 2-dehydrogenase activity; NAD binding; zinc ion binding

Biological Process: carbohydrate metabolic process; fructose biosynthetic process; fructose metabolic process; glucose metabolic process; glucuronate catabolic process to xylulose 5-phosphate; L-xylitol catabolic process; L-xylitol metabolic process; response to cadmium ion; response to copper ion; response to drug; response to hormone stimulus; response to nutrient levels; response to osmotic stress; sorbitol catabolic process; sperm motility