STAT4 recombinant protein

STAT4 recombinant protein

Cat. Num. AAA515122

• Gene Names: STAT4; SLEB11
• Applications: Western Blot
• Synonyms: STAT4; STAT4 recombinant protein; SLEB11

• Host: Sf9 insect cells
• Form/Format: 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Sequence Length: 2840

• Applicable Applications for STAT4 recombinant protein: Kinase Assay, Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: His tag
• Expression System: Sf9 insect cells using baculovirus
• Source Note: Recombinant full-length human STAT4 was expressed by baculovirus in Sf9 insect cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for STAT4 recombinant protein

Recombinant full-length human STAT4 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Scientific Background: STAT4 (signal transducer and activator of transcription 4) is a member of the STAT family that is phosphorylated by receptor-associated protein kinases. STATs form homo- or heterodimers and translocate to the cell nucleus where they act as transcription activators. T helper (Th) cells differentiation is regulated by STAT4 (1). STAT4 controls T1/T2 balance during the response to live influenza virus and isolated viral proteins. The differentiation of gamma interferon (IFN-gamma)-producing Th1 and Tc1 cells after inoculation of live virus occurred independently of STAT4 expression. STAT4 has been shown to control pathogenesis of chronic obstructive pulmonary disease (2).

Product Categories/Family for STAT4 recombinant protein

Transcription Proteins; Signaling Proteins - Transcription Proteins

References

1. Andrea L W. et al: The biology of Stat4 and Stat6. Oncogene, 2000,19: 21, 2577-2584.
2. Cosio. G M.: Autoimmunity, T-cells and STAT-4 in the pathogenesis of chronic obstructive pulmonary disease. Eur Respir J, 2004; 24:3-5.

NCBI and Uniprot Product Information

• NCBI GI #: 345110658
• NCBI GeneID: 6775
• NCBI Accession #: NP_003142.1
• NCBI GenBank Nucleotide #: NM_003151
• UniProt Accession #: Q14765; Q96NZ6
• Molecular Weight: ~87 kDa
• NCBI Official Full Name: Homo sapiens signal transducer and activator of transcription 4 (STAT4), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 4
• NCBI Official Symbol: STAT4
• NCBI Official Synonym Symbols: SLEB11
• NCBI Protein Information: signal transducer and activator of transcription 4
• UniProt Protein Name: Signal transducer and activator of transcription 4
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT4
• UniProt Entry Name: STAT4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]

Uniprot Description

Function: Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.

Subunit structure: Forms a homodimer or a heterodimer with a related family member. Interacts with ARL2BP

By similarity. The SH2 domain interacts, in vitro, with IL12RB2 via a short cytoplasmic domain. Ref.4 Ref.5

Subcellular location: Cytoplasm. Nucleus. Note: Translocated into the nucleus in response to phosphorylation.

Post-translational modification: Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity

By similarity.

Involvement in disease: Systemic lupus erythematosus 11 (SLEB11) [MIM:612253]: A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.6 Ref.8Rheumatoid arthritis (RA) [MIM:180300]: An inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.8

Sequence similarities: Belongs to the transcription factor STAT family.Contains 1 SH2 domain.