Rabbit anti-Human, Mouse MYF5 / MYF 5 Polyclonal Antibody | anti-MYF5 antibody

Rabbit Polyclonal (IgG) to Human MYF5 / MYF 5

Cat. Num. AAA248228

• Gene Names: MYF5; bHLHc2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: MYF5 / MYF 5; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human MYF5 / MYF 5; Anti-MYF5 / MYF 5 Antibody (aa61-110) IHC-plus; MYF5; Myf-5; Myogenic factor 5; BHLHc2; Myogenic factor myf-5; Human MYF5; MYF 5; anti-MYF5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MYF5 Antibody detects endogenous levels of total MYF5 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 255

• Applicable Applications for anti-MYF5 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:40000), IHC-P (30 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human MYF5 (aa61-110).
• Immunogen: MYF5 / MYF 5 antibody was raised against synthetic peptide from human MYF5 (aa61-110).
• Antigen Modification: aa61-110
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Human, Heart: Formalin-Fixed Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot of extracts from HepG2 cells, using MYF5 Antibody. The lane on the right is treated with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 156104906
• NCBI GeneID: 4617
• NCBI Accession #: NP_005584.2
• NCBI GenBank Nucleotide #: NM_005593.2
• UniProt Accession #: P13349; Q6ISR9
• Molecular Weight: 28,296 Da
• NCBI Official Full Name: myogenic factor 5
• NCBI Official Synonym Full Names: myogenic factor 5
• NCBI Official Symbol: MYF5
• NCBI Official Synonym Symbols: bHLHc2
• NCBI Protein Information: myogenic factor 5; myf-5; class C basic helix-loop-helix protein 2
• UniProt Protein Name: Myogenic factor 5
• Protein Family: Myogenic factor
• UniProt Gene Name: MYF5
• UniProt Synonym Gene Names: BHLHC2; Myf-5; bHLHc2
• UniProt Entry Name: MYF5_HUMAN

Uniprot Description

Myf-5: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q21

Cellular Component: nucleoplasm

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein heterodimerization activity

Biological Process: transcription from RNA polymerase II promoter; muscle development; somitogenesis; skeletal muscle development; ossification; extracellular matrix organization and biogenesis; camera-type eye development; regulation of cell-matrix adhesion; muscle cell fate commitment; positive regulation of myoblast differentiation; embryonic skeletal morphogenesis; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; positive regulation of skeletal muscle fiber development; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; cartilage condensation