WWP2 recombinant protein

WWP2 recombinant protein

Cat. Num. AAA515118

• Gene Names: WWP2; AIP2; WWp2-like
• Applications: Western Blot
• Purity: >90%
• Synonyms: WWP2; WWP2 recombinant protein; AIP2; WWp2-like

• Host: Sf9 insect cells
• Purity/Purification: >90%
• Form/Format: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Concentration: 0.1 ug/ul (varies by lot)
• Sequence Length: 4608

• Applicable Applications for WWP2 recombinant protein: Western Blot (WB)
• Accession Number: NM_007014.
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: Sf9 insect cells using baculovirus
• Source Note: Recombinant full-length human WWP2 was expressed by baculovirus in Sf9 insect cells
• Preparation and Storage: Store product at –70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice.

SDS-PAGE

(The purity of WWP2 was determined to be >90% by densitometry. Approx. MW 130 kDa.)

Related Product Information for WWP2 recombinant protein

Recombinant full-length human WWP2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: WWP2 or WW domain containing E3 ubiquitin protein ligase 2 is a member of the Nedd4 family of E3 ligases, which play an important role in protein ubiquitination. WWP2 contains four WW domains and may play a role in multiple processes including chondrogenesis and the regulation of oncogenic signaling pathways via interactions with Smad proteins and the tumor suppressor PTEN. WWP2 is an E3 ubiquitin ligase that targets transcription factor Oct-4 for ubiquitination (1). WWP2 which is also called AIP2 can bind to atrophin-1 (2).

Product Categories/Family for WWP2 recombinant protein

Ubiquitin Proteins; Signaling Proteins - Ubiquitin Proteins

References

1. Xu, H. M. et.al: Wwp2, an E3 ubiquitin ligase that targets transcription factor Oct-4 for ubiquitination. J. Biol. Chem. 279: 23495-23503, 2004.
2. Wood, J. D.et.al: Atrophin-1, the DRPLA gene product, interacts with two families of WW domain-containing proteins. Molec. Cell. Neurosci. 11: 149-160, 1998.

NCBI and Uniprot Product Information

• NCBI GI #: 394581978
• NCBI GeneID: 11060
• NCBI Accession #: NP_008945.2
• NCBI GenBank Nucleotide #: NM_007014
• UniProt Accession #: O00308; Q6ZTQ5; Q96CZ2; Q9BWN6; A6NEP1; B2R706; B4DTL5; F5H213; H3BRF3; I3RSG8
• Molecular Weight: ~130 kDa
• NCBI Official Full Name: Homo sapiens WW domain containing E3 ubiquitin protein ligase 2 (WWP2), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: WW domain containing E3 ubiquitin protein ligase 2
• NCBI Official Symbol: WWP2
• NCBI Official Synonym Symbols: AIP2; WWp2-like
• NCBI Protein Information: NEDD4-like E3 ubiquitin-protein ligase WWP2; atrophin-1 interacting protein 2
• UniProt Protein Name: NEDD4-like E3 ubiquitin-protein ligase WWP2
• Protein Family: NEDD4-like E3 ubiquitin-protein ligase
• UniProt Gene Name: WWP2
• UniProt Synonym Gene Names: AIP2
• UniProt Entry Name: WWP2_HUMAN

NCBI Description

This gene encodes a member of the Nedd4 family of E3 ligases, which play an important role in protein ubiquitination. The encoded protein contains four WW domains and may play a role in multiple processes including chondrogenesis and the regulation of oncogenic signaling pathways via interactions with Smad proteins and the tumor suppressor PTEN. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 10. [provided by RefSeq, Jul 2012]

Uniprot Description

Function: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation. Ref.13 Ref.15

Enzyme regulation: Activated by NDFIP1- and NDFIP2-binding.

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Interacts with POU5F1, RBP1, EGR2 and SLC11A2

By similarity. Interacts with SCNN1A, SCNN1B, SCNN1G, WBP1, WBP2, ATN1 and adenovirus type 2 PIII. Interacts with ERBB4, NDFIP1 AND NDFIP2. Ref.1 Ref.7 Ref.8 Ref.9 Ref.10 Ref.13 Ref.15 Ref.16

Subcellular location: Nucleus Ref.13 Ref.16.

Tissue specificity: Detected in heart, throughout the brain, placenta, lung, liver, muscle, kidney and pancreas. Also detected in spleen and peripheral blood leukocytes. Ref.7 Ref.13

Developmental stage: Highly expressed in undifferentiated embryonic stem cells and expression is reduced after embryoid body (EB) formation. Not detectable at day 13 of EB formation; low levels are again detected at day 18 of EB formation. Ref.13

Domain: The C2 domain is involved in autoinhibition of the catalytic activity by interacting with the HECT domain

By similarity. Ref.12

Post-translational modification: Autoubiquitinated. Ubiquitinated by the SCF(FBXL15) complex, leading to its degradation by the proteasome. Ref.14 Ref.18

Miscellaneous: A cysteine residue is required for ubiquitin-thioester formation.

Sequence similarities: Contains 1 C2 domain.Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.Contains 4 WW domains.

Sequence caution: The sequence BAC86528.1 differs from that shown. Reason: Frameshift at position 757.