Chicken anti-Rat Neurofilament NF-M ck Polyclonal Antibody | anti-NEFM antibody

Anti-Neurofilament M (NF-M)

Cat. Num. AAA502143

• Gene Names: Nefm; Nfm; Nef3
• Reactivity: Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Total IgY fraction
• Synonyms: Neurofilament NF-M ck; Polyclonal Antibody; Anti-Neurofilament M (NF-M); anti-NEFM antibody

• Host: Chicken
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~145k Neurofilament M protein.
• Purity/Purification: Total IgY fraction
• Form/Format: Liquid containing 10mM sodium azide.
• Sequence Length: 845

• Applicable Applications for anti-NEFM antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:5,000; IF: 1:1000
• Antigen: Recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli.
• Immunogen Information: Preparation containing the extreme C-terminus expressed in and purified from E. Coli
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Chicken, human, mouse
• Species Reactivity Note: The antibody has been directly tested for reactivity in chicken, human, mouse and rat tissues.
• Biological Significance: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease (2).
• Preparation and Storage: Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

Testing Data

Related Product Information for anti-NEFM antibody

Chicken polyclonal antibody

References

• Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62.
• Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett 320(3):156-60.

NCBI and Uniprot Product Information

• NCBI GI #: 8393823
• NCBI GeneID: 24588
• NCBI Accession #: NP_058725.1
• NCBI GenBank Nucleotide #: NM_017029.2
• UniProt Accession #: P12839; Q63370
• Molecular Weight: 145
• NCBI Official Full Name: neurofilament medium polypeptide
• NCBI Official Synonym Full Names: neurofilament, medium polypeptide
• NCBI Official Symbol: Nefm
• NCBI Official Synonym Symbols: Nfm; Nef3
• NCBI Protein Information: neurofilament medium polypeptide; NF-M; neurofilament 3, medium; 160 kDa neurofilament protein; neurofilament triplet M protein; Neurofilament protein, middle polypeptide
• UniProt Protein Name: Neurofilament medium polypeptide
• Protein Family: Neurofilament medium polypeptide
• UniProt Gene Name: Nefm
• UniProt Synonym Gene Names: Nef3; Nfm; NF-M
• UniProt Entry Name: NFM_RAT

NCBI Description

neuronal intermediate filament protein; important component of the cytoskeleton [RGD, Feb 2006]

Uniprot Description

Function: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Post-translational modification: There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Ref.4Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization

By similarity. Ref.4

Sequence similarities: Belongs to the intermediate filament family.