Rabbit anti-Rabbit Tryptophan Hydroxylase (Ser58) Polyclonal Antibody | anti-TPH1 antibody

Anti-Phospho-Ser58 Tryptophan Hydroxylase 1

Cat. Num. AAA502129

• Gene Names: Tph1; Tph
• Reactivity: Rabbit
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: Tryptophan Hydroxylase (Ser58); Polyclonal Antibody; Anti-Phospho-Ser58 Tryptophan Hydroxylase 1; anti-TPH1 antibody

• Host: Rabbit
• Reactivity: Rabbit
• Clonality: Polyclonal
• Specificity: Specific for the ~53k tryptophan hydroxylase protein phosphorylated at Ser58.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 444

• Applicable Applications for anti-TPH1 antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser58 of tryptophan Hydroxylase 1 (TPH1).
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 conjugated to KLH
• Immunogen Species: Rat
• Reactivity Assumed Based on 100% Sequence Homology: Bovine chicken, human, mouse non-human primate, rat xenopus zebra fish
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rabbit tissue. It is anticipated that the antibody will react with bovine, canine, human, mouse, non-human primate, rat, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996) Both PKA and CaM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Testing Data

Related Product Information for anti-TPH1 antibody

Affinity purified rabbit polyclonal antibody

References

• Jiang GC, Yohrling GJ, Schmitt JD, Vrana KE (2000) Identification of substrate orienting and phosphorylation sites within tryptophan hydroxylase using homology-based molecular modeling. J Mol Biol 302:1005-1017.
• Johansen PA, Jennings I, Cotton RG, Kuhn DM (1996) Phosphorylation and activation of tryptophan hydroxylase by exogenous protein kinase A. J Neurochem 66:817-823.
• Kuhn, DM, Arthur, Jr, R, States, JC (1997) Phosphorylation and activation of brain tryptophan hydroxylase: identification of serine-58 as a substrate site for protein kinase A. J Neurochem 68:2220-2223.
• Martinez A, Knappskog PM, Haavik J (2001) Structural approach into human tryptophan hydroxylase and its implications for the regulation of serotonin biosynthesis. Curr Med Chem 8:1077-1091.

NCBI and Uniprot Product Information

• NCBI GI #: 158186610
• NCBI GeneID: 24848
• NCBI Accession #: NP_001094104.1
• NCBI GenBank Nucleotide #: NM_001100634.2
• UniProt Accession #: P09810
• Molecular Weight: 55
• NCBI Official Full Name: tryptophan 5-hydroxylase 1
• NCBI Official Synonym Full Names: tryptophan hydroxylase 1
• NCBI Official Symbol: Tph1
• NCBI Official Synonym Symbols: Tph
• NCBI Protein Information: tryptophan 5-hydroxylase 1; tryptophan 5-monooxygenase 1
• UniProt Protein Name: Tryptophan 5-hydroxylase 1
• Protein Family: Tryptophan 5-hydroxylase
• UniProt Gene Name: Tph1
• UniProt Synonym Gene Names: Tph
• UniProt Entry Name: TPH1_RAT

NCBI Description

catalyzes the conversion of l-tryptophan to 5-hydroxy-l-tryptophan; member of pterin-dependent aromatic amino acid hydroxylase family [RGD, Feb 2006]

Uniprot Description

Catalytic activity: L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.

Cofactor: Fe2+ ion.

Pathway: Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.

Subunit structure: Homotetramer.

Sequence similarities: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.Contains 1 ACT domain.