Human Treponema pallidum IgG ELISA Kit
Treponema pallidum (Syphilis) IgG ELISA
Reactivity
Human
Synonyms
Treponema pallidum IgG; Treponema pallidum (Syphilis) IgG ELISA; Treponema pallidum IgG elisa kit
Reactivity
Human
Specificity
The specificity is defined as the probability of the assay of scoring negative in the absence of the specific analyte.
Samples
Human Serum Or Plasma
Preparation and Storage
2 - 8 degree C
Related Product Information for Treponema pallidum IgG elisa kit
Intended Uses: The MyBioSource Schistosoma mansoni IgG-ELISA is intended for the determination of IgG class antibodies against Schistosoma mansoni in human serum or plasma (citrate). For research use only.
Principle of the Assay: The immunoenzymatic determination of IgG-class antibodies against Schistosoma mansoni is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with Schistosoma mansoni antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled Protein A conjugate is added. This conjugate binds to antigen-antibody complexes. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Schistosoma-specific IgG antibodies in the sample. Acidic solution is added to stop the reaction. This produces a yellow endpoint color. Absorbance at 450 nm is read using an ELISA microwell plate reader!!Background/Introduction: Schistosomes belong to the class of distomas (trematodes). They rank among the most frequent pathogens. Estimations originate in more than 200 million affected people. The mature parasites are 6-22 mm long. The most important species are Schistosoma mansoni, S. japonicum and S. haematobium. Schistosoma mansoni is common in Africa, South America and Middle East. Schistosomiasis (bilharziosis) is-depending on species and location of the parasites-a disease of the intestine, liver and spleen resp. urinary passages. Humans are (re)infected by contact with fresh water which is contaminated by ova containing urine or faeces. If larvae bore into human skin, first a transient skin reaction appears (itch with exanthema or erythema, by repeatedly infection cercarial dermatitis is possible). After 3-10 weeks the meanwhile sexually mature worms synthesize cytotoxic and allergic substances which course feverish reaction in humans (Katayama fever). The infected person is mostly harmed by the eggs, which get into organs via blood excreting proteins and glycoproteins. The person reacts under participation of own antibodies and immune complexes with formation of granuloma and granulomatous proliferation in intestine and urinary bladder mucosa. Not excreted eggs die after 3 weeks and will be dissolved or calcified. The affected tissue gets fibrous. In final stage bilharziosis will cause dead.
Principle of the Assay: The immunoenzymatic determination of IgG-class antibodies against Schistosoma mansoni is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with Schistosoma mansoni antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled Protein A conjugate is added. This conjugate binds to antigen-antibody complexes. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Schistosoma-specific IgG antibodies in the sample. Acidic solution is added to stop the reaction. This produces a yellow endpoint color. Absorbance at 450 nm is read using an ELISA microwell plate reader!!Background/Introduction: Schistosomes belong to the class of distomas (trematodes). They rank among the most frequent pathogens. Estimations originate in more than 200 million affected people. The mature parasites are 6-22 mm long. The most important species are Schistosoma mansoni, S. japonicum and S. haematobium. Schistosoma mansoni is common in Africa, South America and Middle East. Schistosomiasis (bilharziosis) is-depending on species and location of the parasites-a disease of the intestine, liver and spleen resp. urinary passages. Humans are (re)infected by contact with fresh water which is contaminated by ova containing urine or faeces. If larvae bore into human skin, first a transient skin reaction appears (itch with exanthema or erythema, by repeatedly infection cercarial dermatitis is possible). After 3-10 weeks the meanwhile sexually mature worms synthesize cytotoxic and allergic substances which course feverish reaction in humans (Katayama fever). The infected person is mostly harmed by the eggs, which get into organs via blood excreting proteins and glycoproteins. The person reacts under participation of own antibodies and immune complexes with formation of granuloma and granulomatous proliferation in intestine and urinary bladder mucosa. Not excreted eggs die after 3 weeks and will be dissolved or calcified. The affected tissue gets fibrous. In final stage bilharziosis will cause dead.
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Product Notes
The Human Treponema pallidum IgG (Catalog #AAA495075) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA495075 ELISA Kit recognizes Human Treponema pallidum IgG. It is sometimes possible for the material contained within the vial of "Treponema pallidum IgG, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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