Typical Testing Data/Standard Curve (for reference only)
Human TIE2 ELISA Kit | TIE2 elisa kit
TIE2 ELISA
Reactivity
Human
Synonyms
TIE2; TIE2 ELISA; TIE2 elisa kit
Reactivity
Human
Samples
Mouse Serum, Plasma, Urine, Tissue Samples Or Cell Culture Supernates
Assay Type
Sandwich
Detection Range
10pg/ml-800pg/ml
Sensitivity
<10pg/ml
Preparation and Storage
2 - 8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TIE2 elisa kit
Intended Uses: For detection of TGF-beta2 in Mouse serum, plasma, urine, tissue samples or cell culture supernates. For research use only.
Principle of the Assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-TGF-beta2 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-TGF-beta2 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TGF-beta2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TGF-beta2 can be calculated!!Background/Introduction: Transforming growth factor-beta 2 (TGF-beta2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. It is known to suppress the effects of interleukin dependent T-cell tumors. It is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma. In 2004, Gottanka found that TGF-beta2 reduced outflow facility when perfused into cultured Mouse anterior segments. Furthermore, TGF-beta2 affected the extracellular matrix of the trabecular meshwork in a manner that was consistent with the observed reduction in outflow facility.
Principle of the Assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-TGF-beta2 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-TGF-beta2 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TGF-beta2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TGF-beta2 can be calculated!!Background/Introduction: Transforming growth factor-beta 2 (TGF-beta2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. It is known to suppress the effects of interleukin dependent T-cell tumors. It is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma. In 2004, Gottanka found that TGF-beta2 reduced outflow facility when perfused into cultured Mouse anterior segments. Furthermore, TGF-beta2 affected the extracellular matrix of the trabecular meshwork in a manner that was consistent with the observed reduction in outflow facility.
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Product Notes
The Human TIE2 (Catalog #AAA494822) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494822 ELISA Kit recognizes Human TIE2. It is sometimes possible for the material contained within the vial of "TIE2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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