WB (Western Blot)
(Detection of human ErbB2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug for WB) cells. Antibodies: Affinity purified rabbit anti-ErbB2 antibody BL3032 (Cat. No. AAA211058) used for WB at 1 ug/ml (A) and 0.04 ug/ml (B) and used for IP at 3 ug/mg lysate. ErbB2 was also immunoprecipitated using rabbit anti-ErbB2 antibodies BL3030 (Cat. No. BL3031 and BL3033 (Cat. No. at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).)
Rabbit anti-Human ErbB2 Polyclonal Antibody | anti-ERBB2 antibody
Rabbit anti-ErbB2 Antibody, Affinity Purified
Gene Names
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
ErbB2, Antibody; Rabbit anti-ErbB2 Antibody, Affinity Purified; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; NGL; p185erbB2; proto-oncogene c-ErbB-2; proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; herstatin; neuroblastoma/glioblastoma derived oncogene homolog; TKR1; HER2; metastatic lymph node gene 19 protein; neuro/glioblastoma derived oncogene homolog; c-erb B2/neu protein; HER-2; HER-2/neu; human epidermal growth factor receptor 2; CD antigen CD340; MLN 19; NEU; CD340; Receptor tyrosine-protein kinase erbB-2; erb-b2 receptor tyrosine kinase 2; anti-ERBB2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-ERBB2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1205 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ErbB2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug for WB) cells. Antibodies: Affinity purified rabbit anti-ErbB2 antibody BL3032 (Cat. No. AAA211058) used for WB at 1 ug/ml (A) and 0.04 ug/ml (B) and used for IP at 3 ug/mg lysate. ErbB2 was also immunoprecipitated using rabbit anti-ErbB2 antibodies BL3030 (Cat. No. BL3031 and BL3033 (Cat. No. at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).)
WB (Western Blot)
(Detection of human ErbB2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug for WB) cells. Antibodies: Affinity purified rabbit anti-ErbB2 antibody BL3032 (Cat. No. AAA211058) used for WB at 1 ug/ml (A) and 0.04 ug/ml (B) and used for IP at 3 ug/mg lysate. ErbB2 was also immunoprecipitated using rabbit anti-ErbB2 antibodies BL3030 (Cat. No. BL3031 and BL3033 (Cat. No. at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).)
IHC (Immunohistochemistry)
(Detection of human ErbB2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ErbB2 (Cat. No. AAA211058 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human ErbB2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ErbB2 (Cat. No. AAA211058 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-ERBB2 antibody
ErbB2 is a member of the receptor tyrosine kinase family of proteins. The four members of this family are ErbB1 (Epidermal growth factor receptor; EGFR/HER1), ErbB2 (HER2/Neu), ErbB3 (HER3), and ErbB4 (HER4). The ErbB receptor members are single-pass transmembrane proteins with an extracellular ligand-binding domain and a cytoplasmic tyrosine-kinase domain. The epidermal growth factor (EGF) and its related proteins serve as the ligands for the ErbB receptors. Upon binding, the receptors heterodimerize, autophosphorylate and make contact with adapter molecules to transmit cellular signals involved in cell proliferation, survival, and differentiation. The ErbB2 receptor is the only member that does not interact with a ligand, but instead functions as a heterodimeric partner to coordinate signals from other ligand bound ErbB receptors. Amplification and/or overexpression of ErbB2 have been reported in many cancers.
Product Categories/Family for anti-ERBB2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
97,253 Da
NCBI Official Full Name
receptor tyrosine-protein kinase erbB-2 isoform a
NCBI Official Synonym Full Names
erb-b2 receptor tyrosine kinase 2
NCBI Official Symbol
ERBB2
NCBI Official Synonym Symbols
NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
NCBI Protein Information
receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; human epidermal growth factor receptor 2; neuro/glioblastoma derived oncogene homolog;
UniProt Protein Name
Receptor tyrosine-protein kinase erbB-2
UniProt Gene Name
ERBB2
UniProt Synonym Gene Names
HER2; MLN19; NEU; NGL; MLN 19
UniProt Entry Name
ERBB2_HUMAN
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Product Notes
The ERBB2 erbb2 (Catalog #AAA211058) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ErbB2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ErbB2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ERBB2 erbb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ErbB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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