Testing Data
(Binding curve of anti-Bet v 1 antibody M0418 (MBS488451) to Bet v 1 ELISA Plate coated with Bet v 1-A (RayBiotech, 228-22389) at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using MBS488451. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used. Absorbance at 450nm does not reach a plateau but falls slightly before increasing once more, as also observed for. This is suggestive of two binding sites on Bet v 1-A for which MBS488451 has differing affinities.)
Bet v 1 Recombinant Antibody | BETVIA recombinant antibody
Anti-Bet v 1 [M0418]
Reactivity
Birch
Applications
ELISA
Purity
Protein A Affinity Purified
Synonyms
Bet v 1; Monoclonal Recombinant Antibody; Anti-Bet v 1 [M0418]; Major Allergen from Birch Pollen; BETVIA recombinant antibody
Reactivity
Birch
Clonality
Monoclonal
Isotype
Rabbit IgG lambda
Clone Number
M0418
Specificity
This antibody binds to Bet v 1, in particular to the naturally occurring isoforms Bet v 1.0101 and Bet v 1.0102, but not to Bet v 1.0112.
Purity/Purification
Protein A Affinity Purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Sequence Length
160
Applicable Applications for BETVIA recombinant antibody
ELISA (EIA), Crystallography
Antibody Type
Recombinant Antibody
Antibody Species
Rabbit
Original Species Name
Human
Immunogen
Bet v 1-A, a major allergen of the European white birch.
Chimeric Use Statement
This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Clone Note
This antibody binds to Bet v 1 via a charaterized epitope and has been used in crystallographic studies.
Buffer Composition
PBS only with 0.02% Proclin 300.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Testing Data
(Binding curve of anti-Bet v 1 antibody M0418 (MBS488451) to Bet v 1 ELISA Plate coated with Bet v 1-A (RayBiotech, 228-22389) at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using MBS488451. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used. Absorbance at 450nm does not reach a plateau but falls slightly before increasing once more, as also observed for. This is suggestive of two binding sites on Bet v 1-A for which MBS488451 has differing affinities.)
Testing Data
(Binding curve of anti-Bet v 1 antibody M0418 (MBS488451) to Bet v 1 ELISA Plate coated with Bet v 1-A (RayBiotech, 228-22389) at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using MBS488451. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used. Absorbance at 450nm does not reach a plateau but falls slightly before increasing once more, as also observed for. This is suggestive of two binding sites on Bet v 1-A for which MBS488451 has differing affinities.)
Related Product Information for BETVIA recombinant antibody
Recombinant monoclonal antibody to Bet v 1. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the single-chain antibody M0418.
Product Categories/Family for BETVIA recombinant antibody
References
Levin et al. Human IgE against the major allergen Bet v 1 – defining an epitope with limited cross-reactivity between different PR-10 family proteins Clin Exp Allergy. 2014 Feb;44(2):288-99. PMID:24447087
Original Published Journal: Clin Exp Allergy. 2014 Feb;44(2):288-99.
Original Published Journal: Clin Exp Allergy. 2014 Feb;44(2):288-99.
Original Published PMID: 24447087
Publication Note: Describes the generation and characterization of the M0418 antibody.
Original Published Journal: Clin Exp Allergy. 2014 Feb;44(2):288-99.
Original Published Journal: Clin Exp Allergy. 2014 Feb;44(2):288-99.
Original Published PMID: 24447087
Publication Note: Describes the generation and characterization of the M0418 antibody.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
NCBI Official Full Name
Major pollen allergen Bet v 1-A
UniProt Protein Name
Major pollen allergen Bet v 1-A
Protein Family
UniProt Gene Name
BETVIA
UniProt Synonym Gene Names
BETVI
Uniprot Description
May be a general steroid carrier protein.
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Product Notes
The BETVIA betvia (Catalog #AAA488451) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Bet v 1 [M0418] reacts with Birch and may cross-react with other species as described in the data sheet. AAA Biotech's Bet v 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Crystallography. Researchers should empirically determine the suitability of the BETVIA betvia for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bet v 1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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