Rabbit anti-Human SYT13 Polyclonal Antibody | anti-SYT13 antibody

SYT13, NT (SYT13, KIAA1427, Synaptotagmin-13, Synaptotagmin XIII) (Azide free) (HRP)

Cat. Num. AAA6352977

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SYT13; Polyclonal Antibody; NT (SYT13; KIAA1427; Synaptotagmin-13; Synaptotagmin XIII) (Azide free) (HRP); Synaptotagmin XIII; anti-SYT13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SYT13 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SYT13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human SYT13.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SYT13 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SYT13 antibody detected the SYT13 protein (arrow).)

Immunohistochemistry (IHC)

(SYT13 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SYT13 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-SYT13 antibody

SYT13 belongs to the large synaptotagmin protein family. All synaptotagmins show type I membrane topology, with an extracellular N terminus, a single transmembrane region, and a cytoplasmic C terminus containing tandem C2 domains. Major functions of synaptotagmins include vesicular traffic, exocytosis, and secretion.

Product Categories/Family for anti-SYT13 antibody

Antibodies; Syntaxin; Synaptotagmin; Synaptophysin

References

Jahn, J.E., et al. Int. J. Oncol. 32(2):441-449(2008). Craxton, M. Genomics 77 (1-2), 43-49 (2001): Fukuda, M., et al. Biochem. J. 354 (PT 2), 249-257 (2001): Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 24308233
• NCBI GeneID: 57586
• NCBI Accession #: NP_065877.1
• NCBI GenBank Nucleotide #: NM_020826.2
• UniProt Accession #: Q7L8C5; Q9BQS3; Q9H041; Q9P2C0; A8K4P4; D3DQP1
• Molecular Weight: 46,885 Da
• NCBI Official Full Name: synaptotagmin-13 isoform 1
• NCBI Official Synonym Full Names: synaptotagmin XIII
• NCBI Official Symbol: SYT13
• NCBI Protein Information: synaptotagmin-13; sytXIII
• UniProt Protein Name: Synaptotagmin-13
• Protein Family: Synaptotagmin
• UniProt Gene Name: SYT13
• UniProt Synonym Gene Names: KIAA1427; SytXIII
• UniProt Entry Name: SYT13_HUMAN

NCBI Description

This gene encodes a member of the large synaptotagmin protein family. Family members have an extracellular N-terminal transmembrane domain and a cytoplasmic C terminus with two tandem C2 domains (C2A and C2B). Synaptotogmin family members can form homo- and heteromeric complexes with each other. They also have different biochemical properties and developmental profiles, and patterns of tissue distribution. Synaptotagmins function as membrane traffickers in multicellular organisms. Two alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

SYT13: May be involved in transport vesicle docking to the plasma membrane. Belongs to the synaptotagmin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: transport vesicle; integral to plasma membrane

Biological Process: vesicle-mediated transport