Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-Integrin beta-7 antibody FIB504 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of FIB504 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Integrin beta-7 Recombinant Antibody | ITGB7 recombinant antibody
Anti-Integrin beta-7 [FIB504]
Gene Names
Itgb7; Ly69
Reactivity
Human, Mouse
Applications
Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Protein A Affinity Purified
Synonyms
Integrin beta-7; Monoclonal Recombinant Antibody; Anti-Integrin beta-7 [FIB504]; FIB-27; FIB 27; Fib-27; Fib27; Fib 27; fib27; fib-27; fib 27; Integrin b7; integrin b-7; Gut homing receptor beta subunit; FIB504; ITGB7 recombinant antibody
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a kappa
Clone Number
FIB504
Specificity
FIB504 recognizes an epitope on intergin beta-7 subunit that overlaps completely with the epitope defined by Fib27 and Fib30, and also partially with the epitope defined by FIB21 and FIB22. In the mouse beta-7 is selectively found on the majority of mature lymphocytes, whereas a small subpopulation of thymocytes and bone marrow cells express beta-7. Beta-7 is also expressed in variable amounts in CD4 T memory cells. Integrin molecules mediate cell-cell and cell-extracellular matrix adhesion and are involved in lymphocyte homing, leukocyte recruitment to inflammatory sites, myogenesis, hemopoiesis, and melanoma metastasis.
Purity/Purification
Protein A Affinity Purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Sequence Length
806
Applicable Applications for ITGB7 recombinant antibody
Immunofluorescence (IF), Flow Cytometry (FC/FACS), Blocking (BL)
Antibody Type
Recombinant Antibody
Antibody Species
Rat
Original Species Name
Rat
Immunogen
FIB504 was prepared by immunizing rats with TK1 cells.
Clone Note
FIB504 can be used for IF, FC and can block TKI cell aggregation and beta-7 intergrin-mediated cell adhesion.
Buffer Composition
PBS only with 0.02% Proclin 300.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-Integrin beta-7 antibody FIB504 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of FIB504 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-Integrin beta-7 antibody FIB504 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of FIB504 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for ITGB7 recombinant antibody
Recombinant monoclonal antibody to Integrin beta-7. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma FIB504.
Product Categories/Family for ITGB7 recombinant antibody
References
Andrew DP, et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J. Immunol. 153:3847 1994 PMID:7523506
Original Published Journal: J. Immunol. 153:3847 1994
Original Published Journal: J. Immunol. 153:3847 1994
Publication Note: Describes the use of FIB504 in defining functional binding regions of alpha-4/beta-7 integrin by epitope mapping.
Original Published Journal: J. Immunol. 153:3847 1994
Original Published Journal: J. Immunol. 153:3847 1994
Publication Note: Describes the use of FIB504 in defining functional binding regions of alpha-4/beta-7 integrin by epitope mapping.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
integrin beta-7
NCBI Official Synonym Full Names
integrin beta 7
NCBI Official Symbol
Itgb7
NCBI Official Synonym Symbols
Ly69
NCBI Protein Information
integrin beta-7
UniProt Protein Name
Integrin beta-7
Protein Family
UniProt Gene Name
Itgb7
UniProt Entry Name
ITB7_MOUSE
Uniprot Description
Function: Integrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is involved in adhesive interactions of leukocytes. It is a receptor for fibronectin and recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Integrin alpha-4/beta-7 is also a receptor for MADCAM1 and VCAM1. It recognizes the sequence L-D-T in MADCAM1. Integrin alpha-E/beta-7 is a receptor for E-cadherin.
Subunit structure: Heterodimer of an alpha and a beta subunit. Beta-7 associates with either alpha-4 or alpha-E.
Subcellular location: Membrane; Single-pass type I membrane protein.
Sequence similarities: Belongs to the integrin beta chain family.Contains 1 VWFA domain.
Research Articles on ITGB7
Similar Products
Product Notes
The ITGB7 itgb7 (Catalog #AAA488442) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Integrin beta-7 [FIB504] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin beta-7 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Flow Cytometry (FC/FACS), Blocking (BL). Researchers should empirically determine the suitability of the ITGB7 itgb7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin beta-7, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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