Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein TMED8 (TMED8) Standard Curve.)
Human Protein TMED8 (TMED8) ELISA Kit | TMED8 elisa kit
Human Protein TMED8 (TMED8) ELISA Kit
Gene Names
TMED8; FAM15B
Reactivity
Human
Synonyms
Protein TMED8 (TMED8); Human Protein TMED8 (TMED8) ELISA Kit; TMED8; FAM15B; MGC126559; family with sequence similarity 15; member B; transmembrane emp24 domain containing 8; TMED8 elisa kit
Reactivity
Human
Specificity
Human Protein TMED8 (TMED8) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMED8. No significant cross-reactivity or interference between Human TMED8 and analogues was observed.
Sequence Length
257
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein TMED8 (TMED8) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein TMED8 (TMED8) Standard Curve.)
Related Product Information for TMED8 elisa kit
Description: This Human Protein TMED8 (TMED8) ELISA Kit employs a two-site sandwich ELISA to quantitate TMED8 in samples. An antibody specific for TMED8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMED8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMED8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMED8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TMED8 contains 1 GOLD domain.Members of the p24 (p24/gp25L/emp24/Erp) family of proteins have been shown to be critical components of the coated vesicles that are involved in the transportation of cargo molecules from the endoplasmic reticulum to the Golgi complex. The p24 proteins form hetero-oligomeric complexes and are believed to function as receptors for specific secretory cargo.The identification of the GOLD domain could aid in directed investigation of the role of the p24 proteins in the secretion process. The newly detected group of GOLD-domain proteins, which might simultaneously bind membranes and other proteins, point to the existence of a novel class of adaptors that could have a role in the assembly of membrane-associated complexes or in regulating assembly of cargo into membranous vesicles.
Background: TMED8 contains 1 GOLD domain.Members of the p24 (p24/gp25L/emp24/Erp) family of proteins have been shown to be critical components of the coated vesicles that are involved in the transportation of cargo molecules from the endoplasmic reticulum to the Golgi complex. The p24 proteins form hetero-oligomeric complexes and are believed to function as receptors for specific secretory cargo.The identification of the GOLD domain could aid in directed investigation of the role of the p24 proteins in the secretion process. The newly detected group of GOLD-domain proteins, which might simultaneously bind membranes and other proteins, point to the existence of a novel class of adaptors that could have a role in the assembly of membrane-associated complexes or in regulating assembly of cargo into membranous vesicles.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein TMED8 isoform c
NCBI Official Synonym Full Names
transmembrane p24 trafficking protein family member 8
NCBI Official Symbol
TMED8
NCBI Official Synonym Symbols
FAM15B
NCBI Protein Information
protein TMED8
UniProt Protein Name
Protein TMED8
Protein Family
UniProt Gene Name
TMED8
UniProt Synonym Gene Names
FAM15B
Uniprot Description
TMED8:
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 14q24.3
Cellular Component: integral component of membrane
Biological Process: transport
Similar Products
Product Notes
The Human TMED8 tmed8 (Catalog #AAA9713968) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713968 ELISA Kit recognizes Human TMED8. It is sometimes possible for the material contained within the vial of "Protein TMED8 (TMED8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
