B7-H3 recombinant antibody
Anti-B7-H3 [MJ18]
Gene Names
Cd276; B7h3; B7RP-2; AU016588; 6030411F23Rik
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Protein A Affinity Purified
Synonyms
B7-H3; Monoclonal Recombinant Antibody; Anti-B7-H3 [MJ18]; B7H3; CD276; B7-H3 recombinant antibody
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG1, Kappa
Clone Number
MJ18
Specificity
This antibody binds to mouse B7-H3 and does not react with other B7-family molecules.
Purity/Purification
Protein A Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
316
Applicable Applications for B7-H3 recombinant antibody
Flow Cytometry (FC/FACS), Block (BL)
Application Notes
This antibody has been used to detect murine B7-H3 by flow cytometry. It blocks B7-H3 signalling and can supress priming of Th2-responses and thereby the development of asthmatic responses in an ovalbumin-induced asthma model in BALB/c mice.
Immunogen
This antibody was generated by immunizing Sprague Dawley rats with B7-H3-Ig, consisting of the extracellular domain (aa1-242 of mouse B7-H3) linked to the Fc portion of mouse IgG2a, emulsified in CFA (Difco). The antibody was selected by its reactivity to mouse B7-H3-transfected cells, but not to parental cells by flow cytometry.
Buffer Composition
PBS only with 0.02% Proclin 300.
Labeled Substance
None
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Related Product Information for B7-H3 recombinant antibody
Recombinant monoclonal antibody to B7-H3. Manufactured using a proprietary Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MJ18.
This antibody was generated by immunizing Sprague Dawley rats with B7-H3-Ig, consisting of the extracellular domain (aa1-242 of mouse B7-H3) linked to the Fc portion of mouse IgG2a, emulsified in CFA (Difco). The antibody was selected by its reactivity to mouse B7-H3-transfected cells, but not to parental cells by flow cytometry.
This antibody was generated by immunizing Sprague Dawley rats with B7-H3-Ig, consisting of the extracellular domain (aa1-242 of mouse B7-H3) linked to the Fc portion of mouse IgG2a, emulsified in CFA (Difco). The antibody was selected by its reactivity to mouse B7-H3-transfected cells, but not to parental cells by flow cytometry.
Product Categories/Family for B7-H3 recombinant antibody
References
Nagashima et al. B7-H3 contributes to the development of pathogenic Th2 cells in a murine model of asthma. J Immunol. 2008 Sep 15; 181(6):4062-71 PMID:18768862
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,001 Da
NCBI Official Full Name
CD276 antigen
NCBI Official Synonym Full Names
CD276 antigen
NCBI Official Symbol
Cd276
NCBI Official Synonym Symbols
B7h3; B7RP-2; AU016588; 6030411F23Rik
NCBI Protein Information
CD276 antigen
UniProt Protein Name
CD276 antigen
UniProt Gene Name
Cd276
UniProt Synonym Gene Names
B7h3; B7-H3
Uniprot Description
Modulates T-cell-mediated immune responses and the development of acute and chronic transplant rejection. Plays a positive regulatory role in bone formation and has a dual role in the bone-immune interface. Induces antitumor immunity as it activates both acquired and innate immunity leading to natural killer cell and CD8 T-cell dependent killing of tumor cells.
Research Articles on B7-H3
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Product Notes
The B7-H3 cd276 (Catalog #AAA488372) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-B7-H3 [MJ18] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's B7-H3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Block (BL). This antibody has been used to detect murine B7-H3 by flow cytometry. It blocks B7-H3 signalling and can supress priming of Th2-responses and thereby the development of asthmatic responses in an ovalbumin-induced asthma model in BALB/c mice. Researchers should empirically determine the suitability of the B7-H3 cd276 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B7-H3, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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