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Western Blot (WB) ( Western Blot using anti-CD4 antibody Ab00167 Mouse thymus (lane 1) and mouse spleen (lane 2) were resolved on a 10% SDS PAGE gel and blots probed with Ab00167-10.0 at 1.5 µg/ml before being detected by a secondary antibody. The expected band size (51 kDa) is seen along with bands corresponding to dimers.)

CD4 recombinant antibody

Anti-CD4 [YNB46.1.8 (Campath-9H)]

Gene Names
CD4; CD4mut
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunohistochemistry
Purity
Protein A Affinity Purified
Synonyms
CD4; Anti-CD4 [YNB46.1.8 (Campath-9H)]; Cluster of differentiation 4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD4 recombinant antibody
Ordering
For Research Use Only!
Reactivity
Human
Isotype
Rat IgG1, Kappa
Clone Number
YNB46.1.8 (Campath-9H)
Specificity
Recognizes the 55kDa human CD4 cell surface antigen, expressed by helper T lymphocytes and by monocytes.
Purity/Purification
Protein A Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
71
Applicable Applications for CD4 recombinant antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunohistochemistry - Frozen (IHC-Fr)
Immunogen
Rat T-cell line (NB2) transfected with the human CD4.
Buffer Composition
PBS only with 0.02% Proclin 300.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Western Blot (WB)

( Western Blot using anti-CD4 antibody Ab00167 Mouse thymus (lane 1) and mouse spleen (lane 2) were resolved on a 10% SDS PAGE gel and blots probed with Ab00167-10.0 at 1.5 µg/ml before being detected by a secondary antibody. The expected band size (51 kDa) is seen along with bands corresponding to dimers.)

Western Blot (WB) ( Western Blot using anti-CD4 antibody Ab00167 Mouse thymus (lane 1) and mouse spleen (lane 2) were resolved on a 10% SDS PAGE gel and blots probed with Ab00167-10.0 at 1.5 µg/ml before being detected by a secondary antibody. The expected band size (51 kDa) is seen along with bands corresponding to dimers.)

Immunohistochemistry (IHC)

( Immunohistochemical staining of rabbit spleen using anti-CD4 antibody (Ab00167) YNB46.1.8 (Campath-9H) Anti-CD4 staining of formaldehyde fixed paraffin embedded rabbit spleen tissue, at 40x magnification. The rat IgG1-chimeric version of YNB46.1.8 (Ab00167-6.1) was used to stain samples at a concentration of 5 µg/ml.)

Immunohistochemistry (IHC) ( Immunohistochemical staining of rabbit spleen using anti-CD4 antibody (Ab00167) YNB46.1.8 (Campath-9H) Anti-CD4 staining of formaldehyde fixed paraffin embedded rabbit spleen tissue, at 40x magnification. The rat IgG1-chimeric version of YNB46.1.8 (Ab00167-6.1) was used to stain samples at a concentration of 5 µg/ml.)
Product Categories/Family for CD4 recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
920
UniProt Accession #
NCBI Official Full Name
CD4, partial
NCBI Official Synonym Full Names
CD4 molecule
NCBI Official Symbol
CD4
NCBI Official Synonym Symbols
CD4mut
NCBI Protein Information
T-cell surface glycoprotein CD4
UniProt Protein Name
CD4 protein
Protein Family
UniProt Gene Name
CD4
UniProt Entry Name
Q13969_HUMAN

NCBI Description

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

Research Articles on CD4

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Product Notes

The CD4 cd4 (Catalog #AAA488137) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD4 [YNB46.1.8 (Campath-9H)] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunohistochemistry - Frozen (IHC-Fr). Researchers should empirically determine the suitability of the CD4 cd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD4, Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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