CD16/CD32 recombinant antibody
Anti-CD16/CD32 [2.4G2]
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
Purity
Protein A Affinity Purified
Synonyms
CD16/CD32; Anti-CD16/CD32 [2.4G2]; Fcgamma III/II Receptor; Low affinity immunoglobulin gamma Fc region receptor III; IgG Fc receptor III; Fc-gamma RIII; FcRIII; CD16; Low affinity immunoglobulin gamma Fc region receptor II; Fc gamma receptor IIB; Fc-gamma RII; Fc-gamma-RIIB; FcRII IgG Fc receptor II beta; Lymphocyte antigen 17; Ly-17; CD32.; CD16/CD32 recombinant antibody
Reactivity
Mouse
Isotype
Rabbit IgG, kappa
Clone Number
2.4G2
Specificity
This antibody binds to an epitope on the extracellular domains of the mouse Fc?III and Fc?II, thereby inhibiting their ability to bind antibodies via their Fc region.
Purity/Purification
Protein A Affinity Purified
Applicable Applications for CD16/CD32 recombinant antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Block
Application Notes
This antibody binds murine Fc Receptors CD16/CD32 and inhibits binding of antibodies to these. It can therefore be used as an Fc-blocking reagent, to reduce non-specific staining of cells by antibodies binding via their Fc-region. A similar effect can be achieved using the Fc Silent range of antibodies that prevent these interactions at the level of the Fc receptor binding site, which has been mutated to lack affinity for the Fc receptors.
Buffer Composition
PBS only with 0.02% Proclin 300.
Immunogen
Mouse BALB/c Macrophage J774 Cell Line.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Related Product Information for CD16/CD32 recombinant antibody
Recombinant monoclonal antibody to CD16/CD32. Manufactured using a Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 2.4G2. This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Product Categories/Family for CD16/CD32 recombinant antibody
References
Unkeless JC. Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors. J Exp Med. 1979 Sep 19; 150(3):580-96. PMID:90108
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Product Notes
The CD16/CD32 (Catalog #AAA488077) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD16/CD32 [2.4G2] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD16/CD32 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Block. This antibody binds murine Fc Receptors CD16/CD32 and inhibits binding of antibodies to these. It can therefore be used as an Fc-blocking reagent, to reduce non-specific staining of cells by antibodies binding via their Fc-region. A similar effect can be achieved using the Fc Silent range of antibodies that prevent these interactions at the level of the Fc receptor binding site, which has been mutated to lack affinity for the Fc receptors. Researchers should empirically determine the suitability of the CD16/CD32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD16/CD32, Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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