GST P1-1 active protein

GST P1-1, Recombinant Human

Cat. Num. AAA480638

• Purity: > 95% as assessed by inspection on a Coomassie® Blue-stained SDS-PAGE gel.
• Synonyms: GST P1-1; Recombinant Human; Recombinant Human GST P1-1; GST P1-1 active protein

• Host: Human
• Purity/Purification: > 95% as assessed by inspection on a Coomassie® Blue-stained SDS-PAGE gel.
• Form/Format: 50 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM DTT, 1 mM EDTA and 50% glycerol.
• Concentration: 1.3615 mg/mL total protein using the Bicinchoninic Acid protein assay (Reducing-agent Compatible) with BSA as a standard. (varies by lot)

• Host Note: Human recombinant protein expressed in E. coli.
• Specific Activity: 91.8625 umol/min/mg (U/mg) chloro-2,4-dinitrobenzene (CDNB) conjugation with reduced glutathione (1 mM) in 100 mM Sodium Phosphate (pH 6.5) at room temperature.
• Preparation and Storage: Store at -20°C. Ships on dry ice. AVOID MULTIPLE FREEZE-THAW CYCLES.

Related Product Information for GST P1-1 active protein

This purified product exhibits high enzymatic activity for CDNB, the synthetic substrate that is most commonly used for GST analyses. However, as is widely known for GST activity analysis using CDNB as a substrate, low concentrations (high dilutions) of the enzyme result in lower activity values. In contrast, initial velocities are much higher for more concentrated levels of enzyme but the rate decreases rapidly. Therefore, activity toward CDNB and these considerations provide guidance when assaying this product under low concentrations or when using substrates other than CDNB.