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Western Blot (WB) (Western Blot analysis of RIDA in Mouse liver tissue and Mouse kidney tissue lysates in order using RIDA Rabbit pAb at dilution 1/650)

Rabbit RIDA Polyclonal Antibody | anti-RIDA antibody

RIDA Rabbit pAb

Gene Names
HRSP12; PSP; P14.5; UK114
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
RIDA; Polyclonal Antibody; RIDA Rabbit pAb; reactive intermediate imine deaminase A homolog; PSP; P14.5; UK114; HRSP12; hp14.5; anti-RIDA antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Concentration
1.2mg/ml (varies by lot)
Applicable Applications for anti-RIDA antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:50-1:200
Immunogen
Fusion protein of human RIDA
Conjugation
Unconjugated
Modification
Unmodified
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of RIDA in Mouse liver tissue and Mouse kidney tissue lysates in order using RIDA Rabbit pAb at dilution 1/650)

Western Blot (WB) (Western Blot analysis of RIDA in Mouse liver tissue and Mouse kidney tissue lysates in order using RIDA Rabbit pAb at dilution 1/650)

Immunohistochemistry (IHC)

(Immunohistochemistry of RIDA in paraffin-embedded Human liver cancer tissue using RIDA Rabbit pAb at dilution 1/70. Predicted cell location: Cytoplasm)

Immunohistochemistry (IHC) (Immunohistochemistry of RIDA in paraffin-embedded Human liver cancer tissue using RIDA Rabbit pAb at dilution 1/70. Predicted cell location: Cytoplasm)
Related Product Information for anti-RIDA antibody
Catalyzes the hydrolytic deamination of enamine/imine intermediates that form during the course of normal metabolism. May facilitate the release of ammonia from these potentially toxic reactive metabolites, reducing their impact on cellular components. It may act on enamine/imine intermediates formed by several types of pyridoxal-5'-phosphate-dependent dehydratases including L-threonine dehydratase. May also function as an endoribonuclease, cleaving mRNA phosphodiester bonds of single-stranded RNA (By similarity). Thereby, may inhibit protein translation (PubMed:8973653).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
137
NCBI Official Full Name
Ribonuclease UK114
NCBI Official Synonym Full Names
heat-responsive protein 12
NCBI Official Symbol
HRSP12
NCBI Official Synonym Symbols
PSP; P14.5; UK114
NCBI Protein Information
ribonuclease UK114; UK114 antigen homolog; translational inhibitor p14.5; perchloric acid-soluble protein; translational inhibitor protein p14.5; 14.5 kDa translational inhibitor protein
UniProt Protein Name
Ribonuclease UK114
UniProt Gene Name
HRSP12
UniProt Synonym Gene Names
PSP; p14.5
UniProt Entry Name
UK114_HUMAN

Uniprot Description

HRSP12: Endoribonuclease responsible for the inhibition of the translation by cleaving mRNA. Inhibits cell-free protein synthesis. Cleaves phosphodiester bonds only in single-stranded RNA. Belongs to the RutC family.

Protein type: Hydrolase; EC 3.1.-.-

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: mitochondrion; cytoplasm; nucleus

Molecular Function: endonuclease activity; deaminase activity

Biological Process: regulation of translational termination

Research Articles on RIDA

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Product Notes

The RIDA hrsp12 (Catalog #AAA4760463) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RIDA Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RIDA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the RIDA hrsp12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RIDA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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