Human S-adenosylmethionine decarboxylase proenzyme ELISA Kit | AMD1 elisa kit

Human S-adenosylmethionine decarboxylase proenzyme ELISA Kit

Cat. Num. AAA760691

• Gene Names: AMD1; AMD; SAMDC; ADOMETDC
• Reactivity: Human
• Synonyms: S-adenosylmethionine decarboxylase proenzyme; Human S-adenosylmethionine decarboxylase proenzyme ELISA Kit; AMD1/AMD (S-adenosylmethionine decarboxylase proenzyme)/AdoMetDC/SAMDC; AMD1 elisa kit

• Reactivity: Human
• Sequence Length: 334

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.625-40ng/ml
• Sensitivity: 0.375ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for AMD1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- AMD1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- AMD1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the AMD1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of AMD1can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 74275346
• NCBI GeneID: 262
• NCBI Accession #: NP_001625.2
• NCBI GenBank Nucleotide #: NM_001634.5
• Molecular Weight: 21,301 Da
• NCBI Official Full Name: S-adenosylmethionine decarboxylase proenzyme isoform 1
• NCBI Official Synonym Full Names: adenosylmethionine decarboxylase 1
• NCBI Official Symbol: AMD1
• NCBI Official Synonym Symbols: AMD; SAMDC; ADOMETDC
• NCBI Protein Information: S-adenosylmethionine decarboxylase proenzyme
• UniProt Protein Name: S-adenosylmethionine decarboxylase proenzyme
• Protein Family: S-adenosylmethionine decarboxylase proenzyme
• UniProt Gene Name: AMD1
• UniProt Synonym Gene Names: AMD; AdoMetDC; SAMDC
• UniProt Entry Name: DCAM_HUMAN

NCBI Description

This gene encodes an important intermediate enzyme in polyamine biosynthesis. The polyamines spermine, spermidine, and putrescine are low-molecular-weight aliphatic amines essential for cellular proliferation and tumor promotion. Multiple alternatively spliced transcript variants have been identified. Pseudogenes of this gene are found on chromosomes 5, 6, 10, X and Y. [provided by RefSeq, Dec 2013]

Uniprot Description

AMD1: an important intermediate enzyme in polyamine biosynthesis. The polyamines spermine, spermidine, and putrescine are low-molecular-weight aliphatic amines essential for cellular proliferation and tumor promotion. Two alternatively spliced transcript variants that encode different proteins have been identified. Pseudogenes of this gene are found on chromosomes 5, 6, 10, X and Y.[provided by RefSeq, Jul 2010]

Protein type: Lyase; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - arginine and proline; EC 4.1.1.50

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytosol

Molecular Function: adenosylmethionine decarboxylase activity; putrescine binding

Biological Process: polyamine metabolic process; S-adenosylmethioninamine biosynthetic process; S-adenosylmethionine metabolic process; spermidine biosynthetic process; spermine biosynthetic process