Western Blot (WB)
(Western Blot analysis of BCAS2 in A172 and 231 cells in order using BCAS2 Rabbit pAb at dilution 1/250)
Rabbit anti-Human, Mouse BCAS2 Polyclonal Antibody | anti-BCAS2 antibody
BCAS2 Rabbit pAb
Gene Names
BCAS2; DAM1; SPF27; Snt309
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
BCAS2; Polyclonal Antibody; BCAS2 Rabbit pAb; breast carcinoma amplified sequence 2; DAM1; SPF27; Snt309; anti-BCAS2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for anti-BCAS2 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:200-1:1000
IHC-P: 1:50-1:200
IHC-P: 1:50-1:200
Immunogen
Fusion protein of human BCAS2
Conjugation
Unconjugated
Modification
Unmodified
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western Blot analysis of BCAS2 in A172 and 231 cells in order using BCAS2 Rabbit pAb at dilution 1/250)
Western Blot (WB)
(Western Blot analysis of BCAS2 in A172 and 231 cells in order using BCAS2 Rabbit pAb at dilution 1/250)
Immunohistochemistry (IHC)
(Immunohistochemistry of BCAS2 in paraffin-embedded Human colon cancer tissue using BCAS2 Rabbit pAb at dilution 1/50. Predicted cell location: Nucleus)
Immunohistochemistry (IHC)
(Immunohistochemistry of BCAS2 in paraffin-embedded Human colon cancer tissue using BCAS2 Rabbit pAb at dilution 1/50. Predicted cell location: Nucleus)
Related Product Information for anti-BCAS2 antibody
BCAS2 (breast carcinoma amplified sequence 2), also designated DAM1 (DNA amplified in mammary carcinoma 1 protein) or spliceosome-associated SPF 27, is a ubiquitously expressed nuclear protein that was originally identified as being overexpressed in various breast cancer cell lines. BCAS2 is now known to be a component of the spliceosome, participating in the removal of introns from mRNA precursors. BCAS2 specifically interacts (in a ligand-independent manner) with TR? (thyroid hormone receptor beta), ERå (estrogen receptor alpha), ER?, PR (progesterone receptor), and PPAR© (Peroxisome proliferator-activated receptor gamma). BCAS2 functions as an ER coactivator and is capable of enhancing ER-mediated transcription. This suggests that BCAS2 is involved in the development of breast cancer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,131 Da
NCBI Official Full Name
pre-mRNA-splicing factor SPF27
NCBI Official Synonym Full Names
breast carcinoma amplified sequence 2
NCBI Official Symbol
BCAS2
NCBI Official Synonym Symbols
DAM1; SPF27; Snt309
NCBI Protein Information
pre-mRNA-splicing factor SPF27; breast carcinoma-amplified sequence 2; spliceosome-associated protein SPF 27; DNA amplified in mammary carcinoma 1 protein; spliceosome associated protein, amplified in breast cancer
UniProt Protein Name
Pre-mRNA-splicing factor SPF27
UniProt Gene Name
BCAS2
UniProt Synonym Gene Names
DAM1
UniProt Entry Name
SPF27_HUMAN
Uniprot Description
BCAS2: Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Belongs to the SPF27 family.
Protein type: RNA splicing; Spliceosome; Nuclear receptor co-regulator; Nucleolus
Chromosomal Location of Human Ortholog: 1p13.2
Cellular Component: nucleoplasm; spliceosome; DNA replication factor A complex; nucleolus; nucleus; cell junction
Molecular Function: protein binding
Biological Process: RNA splicing, via transesterification reactions; nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression
Research Articles on BCAS2
Similar Products
Product Notes
The BCAS2 bcas2 (Catalog #AAA4758900) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BCAS2 Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BCAS2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000 IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the BCAS2 bcas2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCAS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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