Typical Testing Data/Standard Curve (for reference only)
Porcine Luteinizing Hormone-Releasing Hormone ELISA Kit | LHRH elisa kit
Porcine Luteinizing Hormone-Releasing Hormone ELISA Kit
Gene Names
GNRH1; GNRH; LHRH
Reactivity
Porcine
Synonyms
Luteinizing Hormone-Releasing Hormone; Porcine Luteinizing Hormone-Releasing Hormone ELISA Kit; LHRH elisa kit
Reactivity
Porcine
Specificity
This assay has high sensitivity and excellent specificity for detection of LHRH. No significant cross-reactivity or interference between LHRH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LHRH and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LHRH elisa kit
Intended Uses: This LHRH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine LHRH. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: LHRH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-LHRH antibody and an LHRH-HRP conjugate. The assay sample and buffer are incubated together with LHRH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LHRH concentration since LHRH from samples and LHRH-HRP conjugate compete for the anti-LHRH antibody binding site. Since the number of sites is limited, as more sites are occupied by LHRH from the sample, fewer sites are left to bind LHRH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LHRH concentration in each sample is interpolated from this standard curve.
Principle of the Assay: LHRH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-LHRH antibody and an LHRH-HRP conjugate. The assay sample and buffer are incubated together with LHRH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LHRH concentration since LHRH from samples and LHRH-HRP conjugate compete for the anti-LHRH antibody binding site. Since the number of sites is limited, as more sites are occupied by LHRH from the sample, fewer sites are left to bind LHRH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LHRH concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
7,573 Da
NCBI Official Full Name
luteinizing hormone-releasing hormone, partial
NCBI Official Symbol
GNRH1
NCBI Official Synonym Symbols
GNRH; LHRH
NCBI Protein Information
progonadoliberin-1; progonadoliberin I; progonadoliberin-1 preproprotein; luteinizing hormone-releasing hormone
UniProt Protein Name
Progonadoliberin-1
UniProt Gene Name
GNRH1
UniProt Synonym Gene Names
GNRH; LHRH; GnRH-I; LH-RH I
UniProt Entry Name
GON1_MACMU
Uniprot Description
Stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones.
Research Articles on LHRH
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Product Notes
The Porcine LHRH gnrh1 (Catalog #AAA736965) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA736965 ELISA Kit recognizes Porcine LHRH. It is sometimes possible for the material contained within the vial of "Luteinizing Hormone-Releasing Hormone, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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