GST-tag Polyclonal Antibody | anti-GST antibody

Anti-GST-tag-HRP (HRP-Conjugate) Rabbit pAb

Cat. Num. AAA475556

• Reactivity: GST tag from pGEX plasmid
• Applications: Western Blot
• Purity: Affinity Purified
• Synonyms: GST-tag; Polyclonal Antibody; Anti-GST-tag-HRP (HRP-Conjugate) Rabbit pAb; Glutathione S Transferase; Glutathione S transferase Mu 1; Glutathione S-transferase class-mu 26 kDa isozyme; GST 26; GST; GST class mu 1; GST1; GSTM1 1; GSTM1a 1a; GSTM1b 1b; GTH4; GTM1; H B; HB subunit 4; MGC26563; MU 1; MU; SJ26 antigen; SjGST; anti-GST antibody

• Host: Rabbit; Source: Schistosoma japonicum
• Reactivity: GST tag from pGEX plasmid
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: In PBS (pH 7.4) containing with 0.1mg/ml BSA and 50% glycerol.
• Concentration: 0.3mg/ml (varies by lot)

• Applicable Applications for anti-GST antibody: Western Blot (WB)
• Application Notes: WB: 1:5000
• Immunogen: Purified recombinant Escherichia coli BL21 (DE3) GST protein expressed in E Coli.
• Sensitivity: This antibody detects recombinant GST and GST fused proteins and does not cross-react with related proteins.
• Subunit Structure: Protein sequence and structure are important classification methods for GSTs:while classes from the cytosolic superfamily of GSTs possess more than 40% sequence homology, those from other classes may have less than 25%. Cytosolic GSTs are divided into 13 classes based upon their structure:alpha, beta, delta, epsilon, zeta, theta, mu, nu, pi, sigma, tau, phi, and omega.Mitochondrial GSTs are in class kappa.The MAPEG superfamily of microsomal GSTs consists of subgroups designated I-IV, between which amino acid sequences share less than 20% identity.The glutathione binding site, or "G-site, " is located in the thioredoxin-like domain of both cytosolic and mitochondrial GSTs.The region containing the greatest amount of variability between the assorted classes is that of helix alpha2, where one of three different amino acid residues interacts with the glycine residue of glutathione.Two subgroups of cytosolic GSTs have been characterized based upon their interaction with glutathione:the Y-GST group, which uses a tyrosine residue to activate glutathione, and the S/C-GST, which instead use serine or cysteine residues.The porcine pi-class enzyme pGTSP1-1 was the first GST to have its structure determined, and it is representative of other members of the cytosolic GST superfamily, which contain a thioredoxin-like N-terminus domain as well as a C-terminus domain consisting of alpha helices.Mammalian cytosolic GSTs are dimeric, both subunits being from the same class of GSTs, although not necessarily identical.The monomers are in the range of 22-30 kDa.They are active over a wide variety of substrates with considerable overlap.

Western Blot (WB)

(Western blot detection of purified recombinant GST fusion proteins with different molecular weights using GST mouse mAb conjugated with HRP (1:5000 diluted).)

Related Product Information for anti-GST antibody

Entrez Summary: GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. It can provide both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems.

Product Categories/Family for anti-GST antibody

Metabolism; Pathways and Processes; Mitochondrial Metabolism; Mitochondrial markers; Tags & Cell Markers; Fusion/Marker Proteins; GST

NCBI and Uniprot Product Information

• NCBI GI #: 251785093
• NCBI GeneID: 8113787
• NCBI Accession #: YP_002999397
• NCBI GenBank Nucleotide #: NC_012892.2
• Molecular Weight: 26
• NCBI Official Full Name: glutathione S-transferase
• Protein Family: Glutathione S-transferase