Rabbit anti-Human ID2 Polyclonal Antibody | anti-ID2 antibody

ID2 Polyclonal Antibody

Cat. Num. AAA9132018

• Gene Names: Id2; Idb2; C78922; bHLHb26; AI255428
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: ID2; Polyclonal Antibody; ID2 Polyclonal Antibody; AI255428; C78922; Idb2; bHLHb26; anti-ID2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Sequence Length: 134

• Applicable Applications for anti-ID2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500 - 2000
• Immunogen: Recombinant protein of mouse ID2
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ID2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

Related Product Information for anti-ID2 antibody

The protein encoded by this gene belongs to the inhibitor of DNA binding family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the inhibitor of DNA binding family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene of this gene is located on chromosome 3.

Product Categories/Family for anti-ID2 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 8039784
• NCBI GeneID: 15902
• UniProt Accession #: P41136; O88604
• Molecular Weight: 18kDa
• NCBI Official Full Name: DNA-binding protein inhibitor ID-2
• NCBI Official Synonym Full Names: inhibitor of DNA binding 2
• NCBI Official Symbol: Id2
• NCBI Official Synonym Symbols: Idb2; C78922; bHLHb26; AI255428
• NCBI Protein Information: DNA-binding protein inhibitor ID-2
• UniProt Protein Name: DNA-binding protein inhibitor ID-2
• Protein Family: DNA-binding protein inhibitor
• UniProt Gene Name: Id2
• UniProt Synonym Gene Names: Id-2; Idb2

Uniprot Description

ID2: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression. Heterodimer with other HLH proteins. Interacts with GATA4, IFI204 and NKX2-5. Interacts with NR0B2. Highly expressed in early fetal tissues, including those of the central nervous system.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 12 A1.3|12 8.57 cM

Cellular Component: chromatin; cytoplasm; cytosol; nucleus; protein complex

Molecular Function: protein binding

Biological Process: adult locomotory behavior; cell development; cell maturation; circadian regulation of gene expression; circadian rhythm; embryonic digestive tract morphogenesis; entrainment of circadian clock; entrainment of circadian clock by photoperiod; enucleate erythrocyte differentiation; heart development; leukocyte differentiation; locomotor rhythm; lymph node development; mammary gland epithelial cell proliferation; metanephros development; natural killer cell differentiation; negative regulation of B cell differentiation; negative regulation of DNA binding; negative regulation of neuron differentiation; negative regulation of oligodendrocyte differentiation; negative regulation of osteoblast differentiation; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neuron fate commitment; neuron morphogenesis during differentiation; olfactory bulb development; oligodendrocyte development; Peyer's patch development; positive regulation of astrocyte differentiation; positive regulation of blood pressure; positive regulation of cell cycle; positive regulation of cell proliferation; positive regulation of erythrocyte differentiation; positive regulation of fat cell differentiation; positive regulation of macrophage differentiation; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; regulation of circadian rhythm; regulation of lipid metabolic process; thigmotaxis