Western Blot (WB)
(Western blot detection of SESN2 in K562 cell lysates using SESN2 Rabbit mAb(1:1000 diluted). Predicted band size:55kDa. Observed band size:55kDa.)
Rabbit SESN2 Monoclonal Antibody | anti-SESN2 antibody
SESN2 Rabbit mAb
Gene Names
SESN2; HI95; SES2; SEST2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
Affinity purified
Synonyms
SESN2; Monoclonal Antibody; SESN2 Rabbit mAb; Sestrin-2; HI95; SES2; SEST2; anti-SESN2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
R05-8C1
Specificity
Widely expressed.
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-SESN2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
WB: 1:1000
IHC: 1:20
ICC/IF: 1:20
FC/FACS: 1:20
IP: 1:20
IHC: 1:20
ICC/IF: 1:20
FC/FACS: 1:20
IP: 1:20
Immunogen
Recombinant protein of human SESN2
Conjugation
Unconjugated
Modification
Unmodification
Subcellular Location
Cytoplasm
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot detection of SESN2 in K562 cell lysates using SESN2 Rabbit mAb(1:1000 diluted). Predicted band size:55kDa. Observed band size:55kDa.)
Western Blot (WB)
(Western blot detection of SESN2 in K562 cell lysates using SESN2 Rabbit mAb(1:1000 diluted). Predicted band size:55kDa. Observed band size:55kDa.)
Related Product Information for anti-SESN2 antibody
This gene encodes a member of the sestrin family of PA26-related proteins. The encoded protein may function in the regulation of cell growth and survival. This protein may be involved in cellular response to different stress conditions. [provided by RefSeq, Jul 2008]
Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling (PubMed:18692468, PubMed:25263562, PubMed:25457612, PubMed:26449471, PubMed:26612684, PubMed:26586190). Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed:26449471, PubMed:26586190). This stress-inducible metabolic regulator also plays a role in protection against oxidative and genotoxic stresses. May negatively regulate protein translation in response to endoplasmic reticulum stress, via TORC1 (PubMed:24947615). May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed:23274085). May also mediate TP53 inhibition of TORC1 signaling upon genotoxic stress (PubMed:18692468). Has an alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (PubMed:26612684). Was originally reported to contribute to oxidative stress resistance by reducing PRDX1 (PubMed:15105503). However, this could not be confirmed (PubMed:19113821).
Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling (PubMed:18692468, PubMed:25263562, PubMed:25457612, PubMed:26449471, PubMed:26612684, PubMed:26586190). Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed:26449471, PubMed:26586190). This stress-inducible metabolic regulator also plays a role in protection against oxidative and genotoxic stresses. May negatively regulate protein translation in response to endoplasmic reticulum stress, via TORC1 (PubMed:24947615). May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed:23274085). May also mediate TP53 inhibition of TORC1 signaling upon genotoxic stress (PubMed:18692468). Has an alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (PubMed:26612684). Was originally reported to contribute to oxidative stress resistance by reducing PRDX1 (PubMed:15105503). However, this could not be confirmed (PubMed:19113821).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 54 kDa
Observed: 53 kDa
Observed: 53 kDa
NCBI Official Full Name
sestrin-2
NCBI Official Synonym Full Names
sestrin 2
NCBI Official Symbol
SESN2
NCBI Official Synonym Symbols
HI95; SES2; SEST2
NCBI Protein Information
sestrin-2; hypoxia induced gene 95
UniProt Protein Name
Sestrin-2
Protein Family
UniProt Gene Name
SESN2
UniProt Synonym Gene Names
SEST2
UniProt Entry Name
SESN2_HUMAN
NCBI Description
This gene encodes a member of the sestrin family of PA26-related proteins. The encoded protein may function in the regulation of cell growth and survival. This protein may be involved in cellular response to different stress conditions. [provided by RefSeq, Jul 2008]
Uniprot Description
SESN2: Involved in the reduction of peroxiredoxins. Widely expressed. Belongs to the sestrin family.
Protein type: Enzyme, misc.
Chromosomal Location of Human Ortholog: 1p35.3
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding
Biological Process: fatty acid beta-oxidation; glucose import; response to glucose stimulus; protein kinase B signaling cascade; autophagy; mitochondrial DNA metabolic process; response to insulin stimulus
Research Articles on SESN2
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Product Notes
The SESN2 sesn2 (Catalog #AAA4753020) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SESN2 Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SESN2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:1000 IHC: 1:20 ICC/IF: 1:20 FC/FACS: 1:20 IP: 1:20. Researchers should empirically determine the suitability of the SESN2 sesn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SESN2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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