BiP/GRP78 Monoclonal Antibody | anti-BiP/GRP78 antibody

Anti-BiP/GRP78 Mouse mAb

Cat. Num. AAA475249

• Gene Names: HSPA5; BIP; MIF2; GRP78; HEL-S-89n
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Immunoprecipitation
• Purity: Affinity Purified
• Synonyms: BiP/GRP78; Monoclonal Antibody; Anti-BiP/GRP78 Mouse mAb; 78 kDa glucose regulated protein; 78 kDa glucose-regulated protein; AL022860; AU019543; BIP; D2Wsu141e; D2Wsu17e; Endoplasmic reticulum lumenal Ca (2+)-binding protein grp78; Endoplasmic reticulum lumenal Ca2+ binding protein grp78; FLJ26106; Glucose Regulated Protein 78kDa; GRP 78; GRP-78; GRP78; GRP78_HUMAN; Heat shock 70 kDa protein 5; Heat Shock 70kDa Protein 5; Hsce70; HSPA 5; HSPA5; Immunoglobulin Heavy Chain Binding Protein; Immunoglobulin heavy chain-binding protein; mBiP; MIF2; Sez7; anti-BiP/GRP78 antibody

• Host: Mouse; Source: Human
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 4F11-D5-C10
• Purity/Purification: Affinity Purified
• Form/Format: PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
• Concentration: 4.43mg/ml (varies by lot)
• Sequence Length: 654

• Applicable Applications for anti-BiP/GRP78 antibody: Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000
• Immunogen: Purified recombinant human BiP/GRP78 protein expressed in E Coli.
• Sensitivity: This antibody detects endogenous levels of BiP/GRP78 and does not cross-react with related proteins.
• Sequence Similarities: Belongs to the heat shock protein 70 family.
• Subunit Structure: Interacts with DNAJC1 (via J domain) (By similarity). Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with TMEM132A and TRIM21. May form a complex with ERLEC1, OS9, SEL1L and SYVN1. Interacts with DNAJC10 and MX1. Interacts with METTL23. Interacts with CEMIP; the interaction induces calcium leakage from the endoplamic reticulum and cell migration.
• Subcellular Location: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Western Blot (WB)

(Western blot analysis of extracts from 3T3, A549, C6 and Hela cell lysates using BiP/GRP78 mouse mAb (1:1000 diluted).Predicted band size:72KDa.Observed band size:72KDa.)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells fixed fixed by anhydrous methanol at -20 degree C and using BiP/GRP78 mouse mAb (dilution 1:200).)

Immunoprecipitation (IP)

(Immunoprecipitation of BiP/GRP78 from Hela cell extracts using BiP/GRP78 Mouse mAb.Western blot was performed using BiP/GRP78 Mouse mAb.)

Related Product Information for anti-BiP/GRP78 antibody

Entrez Summary: The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.

Product Categories/Family for anti-BiP/GRP78 antibody

Tags & Cell Markers; Subcellular Markers; Organelles; ER

NCBI and Uniprot Product Information

• NCBI GI #: 16507237
• NCBI GeneID: 3309
• NCBI Accession #: NP_005338.1
• NCBI GenBank Nucleotide #: NM_005347
• UniProt Accession #: P11021; Q2EF78; Q9NPF1; Q9UK02; B0QZ61
• Molecular Weight: 78
• NCBI Official Full Name: 78 kDa glucose-regulated protein
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 5
• NCBI Official Symbol: HSPA5
• NCBI Official Synonym Symbols: BIP; MIF2; GRP78; HEL-S-89n
• NCBI Protein Information: 78 kDa glucose-regulated protein
• UniProt Protein Name: 78 kDa glucose-regulated protein
• UniProt Gene Name: HSPA5
• UniProt Synonym Gene Names: GRP78; GRP-78; BiP

NCBI Description

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]

Uniprot Description

GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.

Protein type: Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: cell-cell adherens junction; endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum membrane; ER-Golgi intermediate compartment; extracellular matrix; focal adhesion; integral to endoplasmic reticulum membrane; membrane; midbody; nucleus; signalosome

Molecular Function: ATPase activity; calcium ion binding; chaperone binding; enzyme binding; glycoprotein binding; misfolded protein binding; protein binding; protein domain specific binding; ubiquitin protein ligase binding; unfolded protein binding

Biological Process: cellular response to glucose starvation; ER-associated protein catabolic process; negative regulation of apoptosis; positive regulation of cell migration; substantia nigra development; unfolded protein response