Immunohistochemistry (IHC)
(Fig.1. IHC-P staining of mouse kidney tissue, diluted at 1:200.)
Mouse Ki 67 Monoclonal Antibody | anti-Ki67 antibody
Ki 67 Monoclonal Antibody
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Immunohistochemistry (IHC)
(Fig.1. IHC-P staining of mouse kidney tissue, diluted at 1:200.)
Immunohistochemistry (IHC)
(Fig.1. IHC-P staining of mouse kidney tissue, diluted at 1:200.)
Immunofluorescence (IF)
(Fig.2. Immunofluorescence analysis of human breast cancer tissue. 1, Ki 67 Monoclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
Immunofluorescence (IF)
(Fig.2. Immunofluorescence analysis of human breast cancer tissue. 1, Ki 67 Monoclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
Immunofluorescence (IF)
(Fig.3. Immunofluorescence analysis of mouse testis tissue. 1, Ki 67 Monoclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
Immunofluorescence (IF)
(Fig.3. Immunofluorescence analysis of mouse testis tissue. 1, Ki 67 Monoclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
Immunofluorescence (IF)
(Fig.4. Immunofluorescence analysis of rat brain tissue. 1, Ki 67 Monoclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
Immunofluorescence (IF)
(Fig.4. Immunofluorescence analysis of rat brain tissue. 1, Ki 67 Monoclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
Immunohistochemistry (IHC)
(Fig.5. Immunohistochemical analysis of paraffin-embedded human uterus cancer tissue. 1, Ki 67 Monoclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
Immunohistochemistry (IHC)
(Fig.5. Immunohistochemical analysis of paraffin-embedded human uterus cancer tissue. 1, Ki 67 Monoclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
Immunohistochemistry (IHC)
(Fig.6. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, Ki 67 Monoclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
Immunohistochemistry (IHC)
(Fig.6. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, Ki 67 Monoclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
Uniprot Description
KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers.
Protein type: Nucleolus; Cell cycle regulation
Chromosomal Location of Human Ortholog: 10q26.2
Cellular Component: membrane; cytoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region
Molecular Function: protein C-terminus binding; protein binding; ATP binding
Biological Process: cell proliferation; meiosis
Research Articles on Ki67
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Product Notes
The Ki67 mki67 (Catalog #AAA9700363) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Ki 67 Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ki 67 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF). Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:200). Researchers should empirically determine the suitability of the Ki67 mki67 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ki 67, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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