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Immunohistochemistry (IHC) (Immunohistochemistry (paraffin) Analysis: Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: MBS603600 (1:100) staining pattern/morphology in rat cerebellum. Immunoreactivity is seen as nuclear staining in the neurons in the granular layer. Low Magnification)

Mouse Neuron Specific Nuclear Protein Monoclonal Antibody

Neuron Specific Nuclear Protein (NeuN)

Reactivity
Avian, Chicken, Ferret, Human, Mouse, Porcine, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
Neuron Specific Nuclear Protein; Monoclonal Antibody; Neuron Specific Nuclear Protein (NeuN); Anti -Neuron Specific Nuclear Protein (NeuN); anti-Neuron Specific Nuclear Protein antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Avian, Chicken, Ferret, Human, Mouse, Porcine, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2Q158
Specificity
Recognizes vertebrate neuron-specific nuclear protein (NeuN) at ~46-48kD. Species Crossreactivity: human, rat, mouse, ferret, chicken, salamander, avian, porcine, primate.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Applicable Applications for anti-Neuron Specific Nuclear Protein antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry.
Dilution: Immunohistochemistry (Paraffin): 1:100. Citrate buffer, pH 6.0 may be used for antigen retrieval in rat cerebellum (optimal staining). Immunoreactivity is seen in nuclear staining in neurons in the granular layer. No signal detected in nucleus of Purkinje cells.
Western Blot: Recognizes 2-3 bands in the 46-48kD range and possibly another band at ~66kD.
Immunocytochemistry: 1:10-1:100. Neurons in culture should be permeabilized with 0.1% Triton X-100. N2173 should be diluted in buffer only, without excess protein blocking agents or detergents.
Immunogen
Purified cell nuclei from mouse brain.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemistry (paraffin) Analysis: Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: MBS603600 (1:100) staining pattern/morphology in rat cerebellum. Immunoreactivity is seen as nuclear staining in the neurons in the granular layer. Low Magnification)

Immunohistochemistry (IHC) (Immunohistochemistry (paraffin) Analysis: Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: MBS603600 (1:100) staining pattern/morphology in rat cerebellum. Immunoreactivity is seen as nuclear staining in the neurons in the granular layer. Low Magnification)

Immunohistochemistry (IHC)

(Immunohistochemistry (paraffin) Analysis: Optimal staining with Citrate Buffer, pH 6.0, Epitope Retrieval: MBS603600. Immunolocalization of NeuN (red) and BrdU (green) in the neurogenic regions of the mouse brain (dentate gyrus and subventricular zone).)

Immunohistochemistry (IHC) (Immunohistochemistry (paraffin) Analysis: Optimal staining with Citrate Buffer, pH 6.0, Epitope Retrieval: MBS603600. Immunolocalization of NeuN (red) and BrdU (green) in the neurogenic regions of the mouse brain (dentate gyrus and subventricular zone).)

Testing Data

(Immunolocalization of NeuN (red) using MBS603600 and BrdU (green) in the neurogenic regions of the mouse brain (dentate gyrus and subventricular zone).)

Testing Data (Immunolocalization of NeuN (red) using MBS603600 and BrdU (green) in the neurogenic regions of the mouse brain (dentate gyrus and subventricular zone).)
Related Product Information for anti-Neuron Specific Nuclear Protein antibody
NeuN antibody reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. The immunohistochemical staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. The few cell types which are not reactive include Purkinje, mitral and photoreceptor cells. Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones. The antibody is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants.
Product Categories/Family for anti-Neuron Specific Nuclear Protein antibody

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Product Notes

The Neuron Specific Nuclear Protein (Catalog #AAA603600) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Neuron Specific Nuclear Protein (NeuN) reacts with Avian, Chicken, Ferret, Human, Mouse, Porcine, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neuron Specific Nuclear Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Dilution: Immunohistochemistry (Paraffin): 1:100. Citrate buffer, pH 6.0 may be used for antigen retrieval in rat cerebellum (optimal staining). Immunoreactivity is seen in nuclear staining in neurons in the granular layer. No signal detected in nucleus of Purkinje cells. Western Blot: Recognizes 2-3 bands in the 46-48kD range and possibly another band at ~66kD. Immunocytochemistry: 1:10-1:100. Neurons in culture should be permeabilized with 0.1% Triton X-100. N2173 should be diluted in buffer only, without excess protein blocking agents or detergents. Researchers should empirically determine the suitability of the Neuron Specific Nuclear Protein for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neuron Specific Nuclear Protein, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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