Western Blot (WB)
(Western blot detection of MAGEE1 in K562 cell lysates using MAGEE1 Rabbit mAb(1:1000 diluted). Predicted band size:41kDa. Observed band size:55kDa.)
Rabbit anti-Human MAGEE1 Monoclonal Antibody | anti-MAGEC2 antibody
MAGEE1 Rabbit mAb
Gene Names
MAGEC2; CT10; HCA587; MAGEE1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
Affinity purified
Synonyms
MAGEE1; Monoclonal Antibody; MAGEE1 Rabbit mAb; CT10; HCA587; anti-MAGEC2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
R04-1B9
Specificity
Not expressed in normal tissues, except in germ cells in the seminiferous tubules and in Purkinje cells of the cerebellum. Expressed in various tumors, including melanoma, lymphoma, as well as pancreatic cancer, mammary gland cancer, non-small cell lung cancer and liver cancer. In hepatocellular carcinoma, there is a inverse correlation between tumor differentiation and protein expression, i.e. the lower the differentiation, the higher percentage of expression.
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-MAGEC2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
WB: 1:1000
IHC: 1:20-1:500
ICC/IF: 1:100
FC/FACS: 1:20
IP: 1:20
IHC: 1:20-1:500
ICC/IF: 1:100
FC/FACS: 1:20
IP: 1:20
Immunogen
Recombinant protein of human MAGEE1
Conjugation
Unconjugated
Modification
Unmodification
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot detection of MAGEE1 in K562 cell lysates using MAGEE1 Rabbit mAb(1:1000 diluted). Predicted band size:41kDa. Observed band size:55kDa.)
Western Blot (WB)
(Western blot detection of MAGEE1 in K562 cell lysates using MAGEE1 Rabbit mAb(1:1000 diluted). Predicted band size:41kDa. Observed band size:55kDa.)
Related Product Information for anti-MAGEC2 antibody
Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53 ubiquitination in presence of Ubl-conjugating enzyme UBE2H leading to p53/TP53 degradation. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzymes (E2) at the E3:substrate complex.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,163 Da
NCBI Official Full Name
melanoma-associated antigen C2
NCBI Official Synonym Full Names
melanoma antigen family C, 2
NCBI Official Symbol
MAGEC2
NCBI Official Synonym Symbols
CT10; HCA587; MAGEE1
NCBI Protein Information
melanoma-associated antigen C2; MAGE-C2 antigen; MAGE-E1 antigen; cancer/testis antigen 10; hepatocellular cancer antigen 587; hepatocellular carcinoma-associated antigen 587; melanoma antigen, family E, 1, cancer/testis specific
UniProt Protein Name
Melanoma-associated antigen C2
Protein Family
UniProt Gene Name
MAGEC2
UniProt Synonym Gene Names
HCA587; MAGEE1; CT10
UniProt Entry Name
MAGC2_HUMAN
NCBI Description
This gene is a member of the MAGEC gene family. It is not expressed in normal tissues, except for testis, and is expressed in tumors of various histological types. This gene and the other MAGEC genes are clustered on chromosome Xq26-q27. [provided by RefSeq, Oct 2009]
Uniprot Description
MAGE-C2: Proposed to enhance ubiquitin ligase activity of RING- type zinc finger-containing E3 ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53 ubiquitination in presence of Ubl-conjugating enzyme UBE2H leading to p53/TP53 degradation. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzymes (E2) at the E3:substrate complex.
Protein type: Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: Xq27
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding; ubiquitin protein ligase binding
Biological Process: cellular protein catabolic process; positive regulation of ubiquitin-protein ligase activity
Research Articles on MAGEC2
Similar Products
Product Notes
The MAGEC2 magec2 (Catalog #AAA4752453) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAGEE1 Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAGEE1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:1000 IHC: 1:20-1:500 ICC/IF: 1:100 FC/FACS: 1:20 IP: 1:20. Researchers should empirically determine the suitability of the MAGEC2 magec2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAGEE1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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