Western Blot (WB)
(Western blot detection of SMAD5 (C-terminus) in Hela, Jurkat and K562 cell lysates using SMAD5 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:60KDa.)
anti-Human SMAD5 Monoclonal Antibody | anti-SMAD5 antibody
Anti-SMAD5 (C-term) Mouse mAb
Source: Human
ICC: 1:75
FC: 1:100
Western Blot (WB)
(Western blot detection of SMAD5 (C-terminus) in Hela, Jurkat and K562 cell lysates using SMAD5 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:60KDa.)
Western Blot (WB)
(Western blot detection of SMAD5 (C-terminus) in Hela, Jurkat and K562 cell lysates using SMAD5 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:60KDa.)
Immunocytochemistry (ICC)
(Immunocytochemistry of HeLa cells using anti-SMAD5 (C-terminus) mouse mAb diluted 1:75.)
Immunocytochemistry (ICC)
(Immunocytochemistry of HeLa cells using anti-SMAD5 (C-terminus) mouse mAb diluted 1:75.)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of Jurkat cells stained with SMAD5 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis of Jurkat cells stained with SMAD5 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.)
UniPort Summary Function: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Uniprot Description
SMAD5: transcription factor phosphorylated and activated by bone morphogenetic protein receptor kinases. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where they regulate transcription of target genes. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis.
Protein type: DNA-binding; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; protein complex
Molecular Function: protein binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; ubiquitin protein ligase binding
Biological Process: BMP signaling pathway; embryonic pattern specification
Research Articles on SMAD5
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Product Notes
The SMAD5 smad5 (Catalog #AAA475034) is an Antibody produced from Mouse Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-SMAD5 (C-term) Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMAD5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:1000 ICC: 1:75 FC: 1:100. Researchers should empirically determine the suitability of the SMAD5 smad5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMAD5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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