Mouse anti-Human SMAD5 Monoclonal Antibody | anti-SMAD5 antibody

SMAD5 (C-terminus) Antibody

Cat. Num. AAA9215525

• Gene Names: SMAD5; DWFC; JV5-1; MADH5
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Flow Cytometry, Functional Assay
• Synonyms: SMAD5; Monoclonal Antibody; SMAD5 (C-terminus) Antibody; Purified Mouse Monoclonal Antibody (Mab); DKFZp781C1895; DKFZp781O1323; Dwfc; hSmad 5; hSmad5; JV5 1; JV5-1; MAD homolog 5; MAD mothers against decapentaplegic homolog 5; MAD; mothers against decapentaplegic homolog 5; MADH 5; MADH5; Mothers against decapentaplegic homolog 5; Mothers against DPP homolog 5; MusMLP; SMA and MAD related protein 5; SMAD 5; SMAD family member 5; SMAD mothers against DPP homolog 5; Smad5; SMAD5_HUMAN.; anti-SMAD5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Form/Format: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
• Sequence Length: 465

• Applicable Applications for anti-SMAD5 antibody: Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: WB ~~ 1:1000; ICC ~~ 1:75; FC ~~ 1:100
• Antigen Source: Human
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot detection of SMAD5 (C-terminus) in Hela,Jurkat and K562 cell lysates using SMAD5 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:60KDa.)

Immunocytochemistry (ICC)

(Immunocytochemistry of HeLa cells using anti-SMAD5 (C-terminus) mouse mAb diluted 1:75.)

Flow Cytometry (FC)

(Flow Cytometry analysis of Jurkat cells stained with SMAD5 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.)

Related Product Information for anti-SMAD5 antibody

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

Product Categories/Family for anti-SMAD5 antibody

Cancer; Developmental Biology; Signal Transduction; Stem Cells

References

Riggins G.J.,et al.Nat. Genet. 13:347-349(1996). Hejlik D.P.,et al.Cancer Res. 57:3779-3783(1997). Zavadil J.,et al.Leukemia 11:1187-1192(1997). Gemma A.,et al.Oncogene 16:951-956(1998). Nishimura R.,et al.J. Biol. Chem. 273:1872-1879(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 47778929
• NCBI GeneID: 4090
• NCBI Accession #: NP_001001419.1
• NCBI GenBank Nucleotide #: NM_001001419.2
• UniProt Accession #: Q99717; O14688; Q15798; Q9UQA1
• Molecular Weight: 52258
• NCBI Official Full Name: mothers against decapentaplegic homolog 5
• NCBI Official Synonym Full Names: SMAD family member 5
• NCBI Official Symbol: SMAD5
• NCBI Official Synonym Symbols: DWFC; JV5-1; MADH5
• NCBI Protein Information: mothers against decapentaplegic homolog 5
• UniProt Protein Name: Mothers against decapentaplegic homolog 5
• Protein Family: SMAD5 antisense gene protein
• UniProt Gene Name: SMAD5
• UniProt Synonym Gene Names: MADH5; MAD homolog 5; Mothers against DPP homolog 5; SMAD 5; Smad5; hSmad5
• UniProt Entry Name: SMAD5_HUMAN

NCBI Description

The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

SMAD5: transcription factor phosphorylated and activated by bone morphogenetic protein receptor kinases. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where they regulate transcription of target genes. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; transcription factor complex; protein complex; cytoplasm; integral to membrane; intracellular; nucleus; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding; metal ion binding; receptor signaling protein activity; transcription factor activity; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity

Biological Process: Mullerian duct regression; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; signal transduction; embryonic pattern specification; protein amino acid phosphorylation; germ cell development; BMP signaling pathway; positive regulation of osteoblast differentiation; ureteric bud development; transforming growth factor beta receptor signaling pathway; cartilage development; erythrocyte differentiation; cardiac muscle contraction; osteoblast fate commitment