Western Blot (WB)
(Western blot analysis of RCAN1 expression in human Jurkat (lane 1), rat PC12 (lane 2), human A431 (lane 3), and adult mouse muscle (lane 4). The blot was probed with rabbit polyclonal anti-RCAN1 (C-terminus) at 1:1000.)
Rabbit RCAN1/Dscr1 Polyclonal Antibody | anti-RCAN1C antibody
RCAN1/Dscr1 (C-terminus) Antibody
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA, Immunocytochemistry
Synonyms
RCAN1/Dscr1; Polyclonal Antibody; RCAN1/Dscr1 (C-terminus) Antibody; RCAN1/Dscr1 (C-terminus); anti-RCAN1C antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
The antibody detects several isoforms of RCAN1 on SDS-PAGE immunoblots of adult mouse muscle and brain, rat PC12 cells, and human A431 and Jurkat cells. The predominate isoforms detected were the 38kDa RCAN1L and 31kDa RCAN1S.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-RCAN1C antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
Application Notes
ELISA: 1:2000
ICC: 1:100
WB: 1:1000
ICC: 1:100
WB: 1:1000
Immunogen
RCAN1 (C-terminus) synthetic peptide corresponding to amino acids in the C-terminus of human RCAN1. This sequence is highly conserved in rat and mouse RCAN1, and this sequence is found in all four isoforms of RCAN1, but is not found in RCAN2 or RCAN3.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Western Blot (WB)
(Western blot analysis of RCAN1 expression in human Jurkat (lane 1), rat PC12 (lane 2), human A431 (lane 3), and adult mouse muscle (lane 4). The blot was probed with rabbit polyclonal anti-RCAN1 (C-terminus) at 1:1000.)
Western Blot (WB)
(Western blot analysis of RCAN1 expression in human Jurkat (lane 1), rat PC12 (lane 2), human A431 (lane 3), and adult mouse muscle (lane 4). The blot was probed with rabbit polyclonal anti-RCAN1 (C-terminus) at 1:1000.)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of RCAN1 in aldehyde-fixed NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-RCAN1 (C-terminus) (RP3941) and anti-RCAN1 (a.a. 132-140) (RP3961) antibodies (Left side). These antibodies were also used in the presence (Right side) of blocking peptide RX3945 and RX3965, respectively. The antibodies were detected using appropriate secondary antibody conjugated to DyLight 594.)
Immunocytochemistry (ICC)
(Immunocytochemical labeling of RCAN1 in aldehyde-fixed NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-RCAN1 (C-terminus) (RP3941) and anti-RCAN1 (a.a. 132-140) (RP3961) antibodies (Left side). These antibodies were also used in the presence (Right side) of blocking peptide RX3945 and RX3965, respectively. The antibodies were detected using appropriate secondary antibody conjugated to DyLight 594.)
Related Product Information for anti-RCAN1C antibody
An important element of calcium signaling pathways involves calmodulin activation of calcineurin (phosphatase PP2B), leading to dephosphorylation of transcription factors such as NFAT and MEF2. A wide variety of proteins other than calmodulin have also been implicated in regulating calcineurin activity. Regulators of Calcineurin (RCANs) include RCAN1, RCAN2, and RCAN3. RCAN1 has previously been referred to as Down's syndrome candidate region-1 (Dscr1), MCIP, calcipressin, and Adapt78. This RCAN is expressed as several different variants with RCAN1L (38kDa) and RCAN1S (31kDa) being most prevalent. RCAN1 is increased in Down's syndrome tissues and in a mouse model of Down's syndrome. Increased expression of RCAN1 leads to significant suppression of tumor growth in mice as result of deficits in calcineurin-induced tumor angiogenesis. RCAN1 can recruit TAB1, TAK1, and calcineurin into a macromolecular signaling complex, and TAK1 can phosphorylate Ser-94 and Ser-136 in RCAN1S. This phosphorylation converts RCAN1 from an inhibitor to a facilitator of calcineurin-NFAT signaling.
References
Baek, K.H. et al. (2009) Nature. 459(7250):1126.
Liu, Q. et al. (2009) Nat Cell Biol. 11:154.
Genesca, L. et al. (2003) Biochem J. 374:567.
Liu, Q. et al. (2009) Nat Cell Biol. 11:154.
Genesca, L. et al. (2003) Biochem J. 374:567.
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Product Notes
The RCAN1C (Catalog #AAA474553) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RCAN1/Dscr1 (C-terminus) Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RCAN1/Dscr1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC). ELISA: 1:2000 ICC: 1:100 WB: 1:1000. Researchers should empirically determine the suitability of the RCAN1C for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RCAN1/Dscr1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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