Mouse RasGAP Monoclonal Antibody | anti-RasGAP antibody

RasGAP M335 Antibody

Cat. Num. AAA474550

• Gene Names: RASA1; GAP; PKWS; RASA; CMAVM; CM-AVM; RASGAP; p120GAP; p120RASGAP
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA, Immunocytochemistry
• Synonyms: RasGAP; Monoclonal Antibody; RasGAP M335 Antibody; anti-RasGAP antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M335
• Specificity: This antibody detects a 120kDa* protein corresponding to the molecular mass of RasGAP on SDS-PAGE immunoblots of adult mouse brain, human A431, Jurkat, and SYF fibroblasts.
• Form/Format: Mouse monoclonal, protein A purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-RasGAP antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1:2000; ICC: 1:100; WB: 1:1000
• Immunogen: Clone (M335) was generated from a recombinant sequence in the central region of human RasGAP. This sequence has high homology to mouse and rat RasGAP.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot of mouse brain (lane 1), human A431 (lane 2), Jurkat (lane 3), and SYF fibroblast lysates. The blot was probed with mouse monoclonal anti-RasGAP antibody at 1:1000.)

Immunocytochemistry (ICC)

(Immunocytochemical labeling of RasGap in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal RasGap (RM3351) antibody, then the antibody was detected using appropriate secondary antibody conjugated to DyLight 594. The cells were counterstained with Sytox green to label nuclei.)

Related Product Information for anti-RasGAP antibody

The Ras family (K-Ras, H-Ras, and N-Ras) includes 21kDa guanine-nucleotide binding proteins that cycle between active (GTP-bound) and inactive (GDP-bound) forms. Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAP kinase pathway. Both GTPase-activating proteins (GAPs), such as p120 Ras-GAP, and guanine-nucleotide exchange factors (GEFs) regulate the activity of Ras proteins in many cell signaling pathways. Mutations in Ras are found in several cancers, and the most common oncogenic mutation is Gly12 to Asp (G12D), which prevents Ras inactivation.

References

Ma, J. & Karplus, M. (1997) J. Mol. Biol. 274:114.
Boguski, M.S. & McCormick, F. (1993) Nature 366:643.
Buday, L. & Downward, J. (1993) Cell 73:611.
Huang, D.C. et al. (1993) Mol. Cell Biol. 13:2420.

NCBI and Uniprot Product Information

• NCBI GI #: 121743
• NCBI GeneID: 5921
• UniProt Accession #: P20936; Q9UDI1; B2R6W3
• Molecular Weight: 1047
• NCBI Official Full Name: Ras GTPase-activating protein 1
• NCBI Official Synonym Full Names: RAS p21 protein activator (GTPase activating protein) 1
• NCBI Official Symbol: RASA1
• NCBI Official Synonym Symbols: GAP; PKWS; RASA; CMAVM; CM-AVM; RASGAP; p120GAP; p120RASGAP
• NCBI Protein Information: ras GTPase-activating protein 1; triphosphatase-activating protein
• UniProt Protein Name: Ras GTPase-activating protein 1
• Protein Family: RasGAP-activating-like protein
• UniProt Gene Name: RASA1
• UniProt Synonym Gene Names: GAP; RASA; GAP; GTPase-activating protein; RasGAP
• UniProt Entry Name: RASA1_HUMAN

NCBI Description

The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues. [provided by RefSeq, May 2012]

Uniprot Description

RASA1: a GTPase activator for normal RAS p21 but not its oncogenic counterpart, converting it to the putatively inactive GDP-bound state. Acting as a suppressor of RAS function, it enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; GAPs, Ras; Oncoprotein; GAPs

Chromosomal Location of Human Ortholog: 5q13.3

Cellular Component: ruffle; cytoplasm; cytosol

Molecular Function: GTPase binding; protein binding; glycoprotein binding; potassium channel inhibitor activity; receptor binding

Biological Process: axon guidance; negative regulation of cell adhesion; negative regulation of cell-matrix adhesion; signal transduction; regulation of cell shape; regulation of actin filament polymerization; embryonic development; cytokinesis after mitosis; regulation of RNA metabolic process; ephrin receptor signaling pathway; blood vessel morphogenesis; negative regulation of Ras protein signal transduction; negative regulation of neuron apoptosis; vascular endothelial growth factor receptor signaling pathway; vasculogenesis

Disease: Parkes Weber Syndrome; Capillary Malformation-arteriovenous Malformation; Basal Cell Carcinoma, Susceptibility To, 1