Rabbit anti-Mouse PELP1 Polyclonal Antibody | anti-PELP1 antibody

PELP1, ID (R759) (PELP1, HMX3, MNAR, Proline-, glutamic acid-and leucine-rich protein 1, Modulator of non-genomic activity of estrogen receptor, Transcription factor HMX3) (Biotin)

Cat. Num. AAA6332674

• Gene Names: PELP1; MNAR; P160
• Reactivity: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PELP1; Polyclonal Antibody; ID (R759) (PELP1; HMX3; MNAR; Proline-; glutamic acid-and leucine-rich protein 1; Modulator of non-genomic activity of estrogen receptor; Transcription factor HMX3) (Biotin); Transcription factor HMX3; anti-PELP1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PELP1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PELP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 751-779 amino acids from the Central region of human PELP1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PELP1 Antibody (Center R759) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the PELP1 antibody detected the PELP1 protein (arrow).)

Immunohistochemistry (IHC)

(PELP1 Antibody (Center R759) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PELP1 Antibody (Center R759) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PELP1 antibody

PELP1 is a coactivator of estrogen receptor (see ESR1; MIM 133430)-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors (Choi et al., 2004 [PubMed 15456770]).

Product Categories/Family for anti-PELP1 antibody

Antibodies; Receptors; Estrogen

References

Nair, S.S., et al. EMBO Rep. 11(6):438-444(2010). Habashy, H.O., et al. Breast Cancer Res. Treat. 120(3):603-612(2010). Vadlamudi, R.K., et al. J. Steroid Biochem. Mol. Biol. 118 (4-5), 211-218 (2010): Tzelepi, V., et al. Virchows Arch. 454(1):41-53(2009). Grivas, P.D., et al. Cell. Oncol. 31(3):235-247(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 1527146933
• NCBI GeneID: 27043
• NCBI Accession #: NP_055204
• NCBI GenBank Nucleotide #: NM_014389
• UniProt Accession #: Q8IZL8
• Molecular Weight: 120kDa
• NCBI Official Full Name: proline-, glutamic acid- and leucine-rich protein 1 isoform 1
• NCBI Official Synonym Full Names: proline, glutamate and leucine rich protein 1
• NCBI Official Symbol: PELP1
• NCBI Official Synonym Symbols: MNAR; P160
• NCBI Protein Information: proline-, glutamic acid- and leucine-rich protein 1
• UniProt Protein Name: Proline-, glutamic acid- and leucine-rich protein 1
• Protein Family: Proline-, glutamic acid- and leucine-rich protein
• UniProt Gene Name: PELP1
• UniProt Synonym Gene Names: HMX3; MNAR
• UniProt Entry Name: PELP1_HUMAN

NCBI Description

This gene encodes a transcription factor which coactivates transcription of estrogen receptor responsive genes and corepresses genes activated by other hormone receptors or sequence-specific transcription factors. Expression of this gene is regulated by both members of the estrogen receptor family. This gene may be involved in the progression of several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

PELP1: Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence- specific transcription factors. Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner. Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol. Plays a role in E2-mediated cell cycle progression by interacting with RB1. May have important functional implications in ER/growth factor cross-talk. Interacts with several growth factor signaling components including EGFR and HRS. Involved in nuclear receptor signaling via its interaction with AR and NR3C1. May promote tumorigenesis via its interaction with and modulation of several oncogenes including SRC, PI3K, STAT3 and EGFR. Plays a role in cancer cell metastasis via its ability to modulate E2-mediated cytoskeleton changes and cell migration via its interaction with SRC and PI3K. Interacts with HRS, RXRA, SUMO2, HDAC2, RB1 and STAT3. Interacts with PI3K, SRC and EGFR in cytoplasm. Interacts with ESR1 and ESR2 and this interaction is enhanced by 17-beta- estradiol. Interacts with CREBBP, EP300, AR and NR3C1 in a ligand- dependent manner. Forms two complexes in the presence of 17-beta- estradiol; one with SRC and ESR1 and another with LCK and ESR1. Interacts with histone H1 and H3 with a greater affinity for H1. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Isoform 2 is expressed in breast cancer cell lines. Isoform 1 is widely expressed. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; chromatin binding; transcription factor binding

Biological Process: transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter