Chicken S100 Calcium Binding Protein A8 ELISA Kit | S100A8 elisa kit

Chicken S100 Calcium Binding Protein A8 ELISA Kit

Cat. Num. AAA740006

• Reactivity: Chicken
• Synonyms: S100 Calcium Binding Protein A8; Chicken S100 Calcium Binding Protein A8 ELISA Kit; S100A8 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100A8 elisa kit

Principle of the Assay: S100A8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-S100A8 antibody and an S100A8-HRP conjugate. The assay sample and buffer are incubated together with S100A8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the S100A8 concentration since S100A8 from samples and S100A8-HRP conjugate compete for the anti-S100A8 antibody binding site. Since the number of sites is limited, as more sites are occupied by S100A8 from the sample, fewer sites are left to bind S100A8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A8 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for S100A8 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 47115285
• UniProt Accession #: P05109; Q5SY70; Q9UC84; Q9UC92; Q9UCJ0; Q9UCM6; A8K5L3; D3DV37
• Molecular Weight: 10,835 Da
• NCBI Official Full Name: S100A8
• UniProt Protein Name: Protein S100-A8
• Protein Family: Protein
• UniProt Gene Name: S100A8
• UniProt Synonym Gene Names: CAGA; CFAG; MRP8; CFAG; MRP-8; p8
• UniProt Entry Name: S10A8_HUMAN

Uniprot Description

S100A8: S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH- oxidase by facilitating the enzyme complex assembly at the cell membrane, transfering arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF- kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Belongs to the S-100 family.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; cytoskeleton; plasma membrane; extracellular region; nucleus; cytosol

Molecular Function: arachidonic acid binding; protein binding; RAGE receptor binding; zinc ion binding; microtubule binding; calcium ion binding

Biological Process: caspase activation; neutrophil chemotaxis; chronic inflammatory response; chemokine production; wound healing; cytokine production; response to lipopolysaccharide; positive regulation of peptide secretion; positive regulation of cell growth; leukocyte migration during inflammatory response; activation of NF-kappaB transcription factor; sequestering of zinc ion; response to ethanol; response to zinc ion; defense response to bacterium; autophagy; innate immune response; inflammatory response; defense response to fungus; acute inflammatory response; regulation of cytoskeleton organization and biogenesis; positive regulation of inflammatory response