Mouse anti-Human MAC-2-BP / LGALS3BP Monoclonal Antibody | anti-MAC-2-BP antibody

Mouse Monoclonal [clone 2B1] (IgG1) to Human MAC-2-BP / LGALS3BP

Cat. Num. AAA249101

• Gene Names: LGALS3BP; 90K; M2BP; gp90; CyCAP; BTBD17B; MAC-2-BP; TANGO10B
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A/G Purified
• Synonyms: MAC-2-BP / LGALS3BP; Monoclonal Antibody; Mouse Monoclonal [clone 2B1] (IgG1) to Human MAC-2-BP / LGALS3BP; Anti-MAC-2-BP / LGALS3BP Antibody (clone 2B1) IHC-plus; LGALS3BP; 90K; Galectin-3-binding protein; Mac-2 BP; Mac-2-binding protein; MAC2BP; L3 antigen; Serum protein 90K; TANGO10B; BTBD17B; M2BP; MAC-2-BP; Tumor-associated antigen 90K; Human MAC-2-BP; anti-MAC-2-BP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2B1
• Specificity: Human MAC-2-BP / LGALS3BP
• Purity/Purification: Protein A/G Purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 0.81 mg/ml (varies by lot)
• Sequence Length: 585

• Applicable Applications for anti-MAC-2-BP antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flo (1:100), IHC-P (10 ug/ml), WB (1:2000)
• Target Species: Human
• Immunogen Description: Human recombinant protein fragment corresponding to amino acids19-300 of human LGALS3BP(NP_005558) produced in E. coil.
• Immunogen Type: Recombinant protein
• Immunogen: MAC-2-BP / LGALS3BP antibody was raised against human recombinant protein fragment corresponding to amino acids19-300 of human LGALS3BP(NP_005558) produced in E. coil.
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-MAC-2-BP / LGALS3BP antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Flow Cytometry (FC/FACS)

(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LGALS3BP antibody, and then analyzed by flow cytometry.)

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3BP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3BP.)

Related Product Information for anti-MAC-2-BP antibody

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS3BP has been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV). It appears to be implicated in immune response associated with natural killer (NK) and lymphokine-activated killer (LAK) cell cytotoxicity.

NCBI and Uniprot Product Information

• NCBI GI #: 5031863
• NCBI GeneID: 3959
• NCBI Accession #: NP_005558.1
• NCBI GenBank Nucleotide #: NM_005567.3
• UniProt Accession #: Q08380; Q7M4S0; Q9UCH8; Q9UCH9; Q9UCI0
• Molecular Weight: 65,331 Da
• NCBI Official Full Name: galectin-3-binding protein
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 3 binding protein
• NCBI Official Symbol: LGALS3BP
• NCBI Official Synonym Symbols: 90K; M2BP; gp90; CyCAP; BTBD17B; MAC-2-BP; TANGO10B
• NCBI Protein Information: galectin-3-binding protein; MAC2BP; mac-2 BP; L3 antigen; serum protein 90K; Mac-2-binding protein; tumor-associated antigen 90K; basement membrane autoantigen p105; transport and golgi organization 10 homolog B; lectin galactoside-binding soluble 3-binding protein
• UniProt Protein Name: Galectin-3-binding protein
• UniProt Gene Name: LGALS3BP
• UniProt Synonym Gene Names: M2BP; MAC2BP; Mac-2 BP
• UniProt Entry Name: LG3BP_HUMAN

NCBI Description

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS3BP has been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV). It appears to be implicated in immune response associated with natural killer (NK) and lymphokine-activated killer (LAK) cell cytotoxicity. Using fluorescence in situ hybridization the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds galectin 1. [provided by RefSeq, Jul 2008]

Uniprot Description

LGALS3BP: Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; membrane

Molecular Function: scavenger receptor activity

Biological Process: receptor-mediated endocytosis; cellular defense response; signal transduction; cell adhesion