Rabbit Semaphorin-3F Polyclonal Antibody | anti-SEMA3F antibody

Semaphorin-3F (a.a. 746-762)

Cat. Num. AAA474142

• Gene Names: SEMA3F; SEMA4; SEMAK; SEMA-IV
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA
• Purity: Affinity Purified
• Synonyms: Semaphorin-3F; Polyclonal Antibody; Semaphorin-3F (a.a. 746-762); SemaphorinIV; SemaIV; SemaIII/F; anti-SEMA3F antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects a 95 kDa* protein corresponding to the apparent molecular mass of Sema-3F on SDS-PAGE immunoblots of adult mouse brain and liver.
• Purity/Purification: Affinity Purified
• Form/Format: Supplied in 100ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
• Sequence Length: 785

• Applicable Applications for anti-SEMA3F antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA: 1:2000; WB: 1:1000; WB: Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
• Immunogen: Semaphorin 3F synthetic peptide (coupled to carrier protein) corresponds to amino acids 746 to 762 in human Sema-3F. This sequence is highly conserved in rat and mouse Sema-3F, and has low homology to other semaphorin family members.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot analysis of Sema-3F expression in adult mouse brain (lane 1), kidney (lane 2), and liver (lane 3). The blot was probed with anti-Sema-3F (SP3921) at 1:1000. )

Related Product Information for anti-SEMA3F antibody

One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that have been implicated in neuron development, vascular disease, and tumor progression. There are eight classes of semaphorin genes, all of which are characterized by a conserved 500 amino acid, cystine-rich Sema domain. Semaphorin 3F (Sema-3F) is a class III secreted semaphorin that binds with high affinity to Neuropilin-2, and low affinity to Neuropilin-1. During peripheral and central nervous system development, Sema-3F has critical roles in axon guidance and dendrite outgrowth. Sema-3F has also been shown to inhibit angiogenesis by manipulating VEGFR function and decreasing blood vessel density. In cancers, Sema-3F induces a poorly vascularized, encapsulated, non-metastatic phenotype through chemorepulsion of endothelial cells in melanoma, while Sema-3F disrupts intercellular contacts of MCF7 breast cancer cells through delocalization of E-cadherin and beta-catenin. Thus, Sema-3F may have important roles in axon guidance, angiogenesis, and tumor progression.

References

Xiang, R.H. (1996) Genomics. 32(1):39.Kolk, S.M. et al. (2009) J Neurosci. 29(40):12542.Tran, T.S. et al. (2009) Nature. 462(7276):1065.

NCBI and Uniprot Product Information

• NCBI GI #: 188497703
• NCBI GeneID: 6405
• NCBI Accession #: NP_004177.3
• NCBI GenBank Nucleotide #: NM_004186.5
• UniProt Accession #: Q13275
• Molecular Weight: 95 kDa
• NCBI Official Full Name: semaphorin-3F isoform 1
• NCBI Official Synonym Full Names: semaphorin 3F
• NCBI Official Symbol: SEMA3F
• NCBI Official Synonym Symbols: SEMA4; SEMAK; SEMA-IV
• NCBI Protein Information: semaphorin-3F
• UniProt Protein Name: Semaphorin-3F
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA3F
• UniProt Synonym Gene Names: Sema IV

NCBI Description

This gene encodes a member of the semaphorin III family of secreted signaling proteins that are involved in axon guidance during neuronal development. The encoded protein contains an N-terminal Sema domain, an immunoglobulin loop and a C-terminal basic domain. This gene is expressed by the endothelial cells where it was found to act in an autocrine fashion to induce apoptosis, inhibit cell proliferation and survival, and function as an anti-tumorigenic agent. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]

Uniprot Description

May play a role in cell motility and cell adhesion.