Cofilin 1 + LIMK1 Lysate

Applications

Western Blot

Synonyms

Cofilin 1 + LIMK1; Cofilin 1 + LIMK1 Lysate; Cofilin 1 + LIMK1 lysate

Ordering
Cat. Num. AAA474089 For Research Use Only!

Form/Format

Supplied at a concentration of 10 ng/ul in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% beta-mercaptoethanol).

Applicable Applications for Cofilin 1 + LIMK1 lysate

Western Blot (WB)

Application Notes

WB: 10 mul/lane

Lysate Preparation

Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cell migration, cytokinesis, axon development, and immune cell activation. In mammals, there are three members of the AC family, muscle-specific cofilin (cofilin 2), non-muscle cofilin (cofilin 1), and ADF. In humans, cofilin 1 and ADF have 72% identity, with the major amino acid differences found in the C-terminal region. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo.
For preparation of Ser-3 phosphorylated Cofilin, purified human Cofilin 1 was phosphorylated in vitro with His-tagged mouse recombinant LIM Kinase 1 for 30 min. at 37 degree C. For control lysate, the recombinant Cofilin 1 protein was exposed to buffer only. The phosphorylated product is detected by anti-Cofilin (Ser-3), while both the control and phosphorylated recombinant cofilin 1 are detected by anti-Cofilin (Nterminus).

Preparation and Storage

Store at -20 degree C. Do not boil or dilute. Stable for 1 year.

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Product Notes

The Cofilin 1 + LIMK1 (Catalog #AAA474089) is a Lysate and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cofilin 1 + LIMK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 10 mul/lane. Researchers should empirically determine the suitability of the Cofilin 1 + LIMK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cofilin 1 + LIMK1, Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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Cofilin 1 + LIMK1 Lysate