phospho-p130 Cas (Tyr-751) Peptide
phospho-p130 Cas (Tyr-751) Blocking Peptide
Synonyms
phospho-p130 Cas (Tyr-751) Peptide; phospho-p130 Cas (Tyr-751) Blocking Peptide; phospho-p130 Cas (Tyr-751) Peptide peptide
Specificity
The peptide is specifically recognized by anti-p130 Cas (Tyr-751) phospho-specific antibody (PP1581) in ELISA, and has been shown to block the reactivity of PP1581 during Western blot. In addition, the peptide is recommended for use in blocking PP1581 reactivity in immunocytochemistry.
Form/Format
Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.
Sequence
Peptide Sequence: Phospho-p130 Cas (Tyr-751) synthetic peptide corresponds to amino acid residues around tyrosine 751 in rat p130 Cas. This rat sequence is the same as Tyr-653 in human p130 Cas and Tyr-653 in mouse p130 Cas. This site is not conserved in other Cas family members.
Application Notes
Antibody Blocking: 1 ug/ml
ELISA: 10-100 ng/well
ELISA: 10-100 ng/well
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Related Product Information for phospho-p130 Cas (Tyr-751) Peptide peptide
p130 Cas (Crk-associated substrate (CAS), breast cancer antiestrogen resistance 1 (BCAR1)) is a docking protein containing multiple protein-protein interaction domains. The amino-terminal SH3 domain may function as a molecular switch regulating CAS tyrosine phosphorylation, as it interacts with focal adhesion kinase (FAK) and the FAK-related kinase PYK2, as well as with the tyrosine phosphatases PTP-1B and PTP-PEST. The carboxy-terminal Src binding domain (SBD) contains a prolinerich motif that mediates interaction with the SH3 domains of Src-family kinases (SFKs) and a tyrosine phosphorylation site (Tyr-762 in rat or Tyr-668 in mouse p130 Cas) that can promote interaction with the SH2 domain of SFKs. The p130 Cas central substrate domain, the major region of tyrosine phosphorylation, is characterized by 15 tyrosines present in Tyr-X-Xpro (YXXP) motifs, including Tyr-165, Tyr-249, and Tyr-410. When phosphorylated, most YXXP motifs are able to serve as docking sites for proteins with SH2 or PTB domains, including the adaptors Crk and Nck. In addition, phosphorylation of Tyr- 751 (Tyr-657 in human) near the C-terminal caspase recognition site can attenuate caspase cleavage. Dephosphorylation of this site occurs during apoptosis, and may facilitate p130 Cas degradation.
References
• Nakamoto, T. et al. (1996) J Biol Chem. 271(15):8959.
• Pellicena, P. & Miller, W.T. (2001) J Biol Chem. 276 (30):28190.
• Kim, D.H. et al. (2003). Biochem Biophys Res Comm. 300:141.
• Shin, N-Y et al. (2004). J Biol Chem. 279(37):38331.
• Pellicena, P. & Miller, W.T. (2001) J Biol Chem. 276 (30):28190.
• Kim, D.H. et al. (2003). Biochem Biophys Res Comm. 300:141.
• Shin, N-Y et al. (2004). J Biol Chem. 279(37):38331.
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Product Notes
The phospho-p130 Cas (Tyr-751) Peptide (Catalog #AAA474046) is a Peptide and is intended for research purposes only. The product is available for immediate purchase. Antibody Blocking: 1 ug/ml ELISA: 10-100 ng/well. Researchers should empirically determine the suitability of the phospho-p130 Cas (Tyr-751) Peptide for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Peptide Sequence: Phospho-p1 30 Cas (Tyr-751) synthetic peptide correspond s to amino acid residues around tyrosine 751 in rat p130 Cas. This rat sequence is the same as Tyr-653 in human p130 Cas and Tyr-653 in mouse p130 Cas. This site is not conserved in other Cas family members. It is sometimes possible for the material contained within the vial of "phospho-p130 Cas (Tyr-751) Peptide, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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