Testing Data
Rabbit p130 Cas (Tyr-762) Polyclonal Antibody
Anti-p130 Cas (Tyr-762), phospho-specific
Reactivity
Human, Rat, Mouse
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
Affinity Purified
Synonyms
p130 Cas (Tyr-762); Polyclonal Antibody; Anti-p130 Cas (Tyr-762); phospho-specific; BCAR1; Breast cancer anti-estrogen resistance protein 1; CRK associated substrate; Cas; anti-p130 Cas (Tyr-762) antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-p130 Cas (Tyr-751) peptide before affinity purification using phospho-p130 Cas (Tyr-762) peptide (without carrier). The antibody detects a 130 kDa* protein corresponding to the molecular mass of phosphorylated p130 Cas on SDS-PAGE immunoblots of pervanadate treated human A431 cells, but not in control cells.
Purity/Purification
Affinity Purified
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-p130 Cas (Tyr-762) antibody
ELISA, Western Blot, Immunoprecipitation
Application Notes
ELISA: 1:2000
Immunoprecipitation: 5-10ml
Western Blot: 1:2000
Immunoprecipitation: 5-10ml
Western Blot: 1:2000
Immunogen
Phospho-p130 Cas (Tyr-762) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 762 in the Src-binding domain of rat p130 Cas. This rat sequence is the same as Tyr-664 in human p130 Cas and Tyr-668 in mouse p130 Cas. This site is also highly conserved in Cas-L (HEF1, Nedd9).
Preparation and Storage
Store at -20 degree C. Do not aliquot. Stable for 1 year.
Testing Data
Testing Data
Related Product Information for anti-p130 Cas (Tyr-762) antibody
p130 Cas (Crk-associated substrate (CAS), breast cancer antiestrogen resistance 1 (BCAR1)) is a docking protein containing multiple protein-protein interaction domains. The amino-terminal SH3 domain may function as a molecular switch regulating CAS tyrosine phosphorylation, as it interacts with focal adhesion kinase (FAK) and the FAK-related kinase PYK2, as well as with the tyrosine phosphatases PTP-1B and PTP-PEST. The carboxy-terminal Src binding domain (SBD) contains a proline-rich motif that mediates interaction with the SH3 domains of Src-family kinases (SFKs) and a tyrosine phosphorylation site (Tyr-762 in rat or Tyr-668 in mouse p130 Cas) that can promote interaction with the SH2 domain of SFKs. The p130 Cas central substrate domain, the major region of tyrosine phosphorylation, is characterized by 15 tyrosines present in Tyr-X-X-Pro (YXXP) motifs, including Tyr-165, Tyr-249, and Tyr-410. When phosphorylated, most YXXP motifs are able to serve as docking sites for proteins with SH2 or PTB domains, including the adaptors Crk and Nck. In addition, phosphorylation of Tyr-751 (Tyr-653 in human) near the C-terminal caspase recognition site can attenuate caspase cleavage. Dephosphorylation of this site occurs during apoptosis, and may facilitate p130 Cas degradation.
References
• Nakamoto, T. et al. (1996) J Biol Chem. 271 (15):8959.
• Pellicena, P. & Miller, W.T. (2001) J Biol Chem. 276 (30):28190.
• Kim, D.H. et al. (2003) Biochem Biophys Res Comm. 300:141.
• Shin, N.-Y. et al. (2004) J Biol Chem. 279 (37):38331.
• Pellicena, P. & Miller, W.T. (2001) J Biol Chem. 276 (30):28190.
• Kim, D.H. et al. (2003) Biochem Biophys Res Comm. 300:141.
• Shin, N.-Y. et al. (2004) J Biol Chem. 279 (37):38331.
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Product Notes
The p130 Cas (Tyr-762) (Catalog #AAA472043) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-p130 Cas (Tyr-762), phospho-specific reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's p130 Cas (Tyr-762) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunoprecipitation. ELISA: 1:2000 Immunoprecipitation: 5-10ml Western Blot: 1:2000. Researchers should empirically determine the suitability of the p130 Cas (Tyr-762) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p130 Cas (Tyr-762), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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