Rabbit AMPK-alpha Polyclonal Antibody | anti-AMPK-alpha antibody

Rabbit anti AMPK-alpha Polyclonal Antibody

Cat. Num. AAA460499

• Reactivity: Human, Rat, Mouse, Bovine, Chicken, Dog
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Epitope Affinity Purification
• Synonyms: AMPK-alpha; Polyclonal Antibody; Rabbit anti AMPK-alpha Polyclonal Antibody; AMPKalpha; AMP-activated protein kinase alpha; AMPK; PRKAA1; anti-AMPK-alpha antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse, Bovine, Chicken, Dog
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Epitope Affinity Purification
• Form/Format: Supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.

• Applicable Applications for anti-AMPK-alpha antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Flow Cytometry: 5-10 ug/ml; Immunohistochemistry: 2-10 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml
• Preparation: A synthetic peptide surrounding to the epitope -LRTSC- with a phosphorylation site at Thr 172 of AMPK alpha protein from human origin
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Related Product Information for anti-AMPK-alpha antibody

AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy balance at both the cellular and the whole body levels. Once activated, it affects a metabolic switch from an anabolic to a catabolic state, both by acutely phosphorylating metabolic enzymes and, in the longer term, by regulating gene expression. AMPK is a heterotrimeric complex composed of a catalytic a subunit and regulatory beta and ? subunits. Binding APM to the beta domains triggers increased phosphorylation at Thr172 on the activation loop of the alpha subunit. AMP Phosphorylation at Thr172 is catalyzed by the tumor suppressor kinase LKB1 or CaMKK-beta, TGF-beta activated kinase-1 (TAK1). AMPKa is also phosphorylated at Thr258 and Ser485 (for a1; Ser491 for a2).

References

Jing Chen et al. AMPK regulation of mouse oocyte meiotic resumption in vitro. Developmental Biology, 291 (2), 227-238 (2006).