Rabbit anti-Human TSEN2 Polyclonal Antibody | anti-TSEN2 antibody

TSEN2 Antibody

Cat. Num. AAA1499838

• Gene Names: TSEN2; SEN2; PCH2B; SEN2L
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Antigen Affinity Purified
• Synonyms: TSEN2; Polyclonal Antibody; TSEN2 Antibody; tRNA-intron endonuclease Sen2; HsSen2; SEN2; anti-TSEN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 465

• Applicable Applications for anti-TSEN2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Recommended dilution: WB:1:200-1000
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human tRNA-splicing endonuclease subunit Sen2 protein (1-220aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-103297 / sc-103299
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: TSEN2 antibody at 5ug/ml+293T whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 54,52,50,48,46 kDaObserved band size: 53 kDa)

Related Product Information for anti-TSEN2 antibody

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity.

NCBI and Uniprot Product Information

• NCBI GI #: 223972632
• NCBI GeneID: 80746
• NCBI Accession #: NP_001138864.1
• NCBI GenBank Nucleotide #: NM_001145392.1
• UniProt Accession #: Q8NCE0; Q8WTW7; Q9BPU7; B7Z6K1; C9IZI7; G5E9Q3
• Molecular Weight: 45,532 Da
• NCBI Official Full Name: tRNA-splicing endonuclease subunit Sen2 isoform 1
• NCBI Official Synonym Full Names: tRNA splicing endonuclease subunit 2
• NCBI Official Symbol: TSEN2
• NCBI Official Synonym Symbols: SEN2; PCH2B; SEN2L
• NCBI Protein Information: tRNA-splicing endonuclease subunit Sen2
• UniProt Protein Name: tRNA-splicing endonuclease subunit Sen2
• UniProt Gene Name: TSEN2
• UniProt Synonym Gene Names: SEN2; HsSen2
• UniProt Entry Name: SEN2_HUMAN

NCBI Description

This gene encodes one of the subunits of the tRNA splicing endonuclease. This endonuclease catalyzes the first step in RNA splicing which is the removal of introns. Mutations in this gene have been associated with pontocerebellar hypoplasia type 2. A pseudogene has been identified on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2009]

Uniprot Description

SEN2: Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre- mRNA 3'-end processing factors, establishing a link between pre- tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity. Defects in TSEN2 are the cause of pontocerebellar hypoplasia type 2B (PCH2B). Pontocerebellar hypoplasia (PCH) is a heterogeneous group of disorders characterized by an abnormally small cerebellum and brainstem. PCH type 2 is characterized by progressive microcephaly from birth combined with extrapyramidal dyskinesia and chorea, epilepsy, and normal spinal cord findings. Belongs to the tRNA-intron endonuclease family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ribonuclease; EC 4.6.1.16; Nucleolus; RNA processing

Chromosomal Location of Human Ortholog: 3p25.2

Cellular Component: centrosome; cytoplasm; nucleolus; nucleoplasm; tRNA-intron endonuclease complex

Molecular Function: lyase activity; nucleic acid binding; protein binding; tRNA-intron endonuclease activity

Biological Process: gene expression; mRNA processing; tRNA processing; tRNA splicing; tRNA-type intron splice site recognition and cleavage

Disease: Pontocerebellar Hypoplasia, Type 2b