Rabbit TGF beta R1 Polyclonal Antibody | anti-TGF beta R1 antibody

Rabbit anti TGF beta R1 Polyclonal Antibody

Cat. Num. AAA460088

• Gene Names: Tgfbr1; Alk5; Skr4; Tgfr-1; tbetaR-I
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: The Rabbit IgG is purified by Epitope Affinity Purification.
• Synonyms: TGF beta R1; Polyclonal Antibody; Rabbit anti TGF beta R1 Polyclonal Antibody; Transforming Growth Factor beta Receptor Type 1 (TGFBR1); ALK5; anti-TGF beta R1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody recognizes TGFbetaR1protein. This antibody reacts with human, rat and mouse. The other species are not tested.
• Purity/Purification: The Rabbit IgG is purified by Epitope Affinity Purification.
• Form/Format: This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer
• Sequence Length: 497

• Applicable Applications for anti-TGF beta R1 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Western Blot: 0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml; IHC: Not tested ; Flow cytometry: Not tested; Optimal dilutions should be determined by researchers for the specific applications.
• Preparation: A synthetic peptide derived from internal epitope (180aa-210aa) of human TGFbR1 protein
• Antigen Preparation: A synthetic peptide derived from internal sequence (180-210aa) of human TGFbeta receptor type-1. This sequence is also identical within human, rat, mouse, chicken, bovine and dog species.
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at –20 degree C to –70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Western Blot

( The cell lysate derived from HEK293 transfected with full length of TGFbetaR1 was immunoprobed by Rabbit anti-TGFbetaR1 (Cat#MBS460088) at 1:500. An immunoreactive band is observed around ~55 kDa)

Related Product Information for anti-TGF beta R1 antibody

Transforming growth factor ? isoforms (TGF-?1, 2, 3) play vital roles in regulating cellular growth and differentiation, and they signal through a highly restricted subset of receptors known as TGF-? type I receptor (T?R-I) and TGF-? type II receptor (T?R-II). TGF-?'s specificity for T?R-I has been proposed to arise from its pre-helix extension, a five-residue loop that binds in the cleft between TGF-? and T?R-II. TGF-? binds with high affinity to the type II receptor, a transmembrane protein with a cytoplasmic serine/threonine kinase domain, which requires both its kinase activity and association with another TGF beta-binding protein, the type I receptor, to signal growth inhibition and early gene responses. Receptors I and II associate as interdependent components of a heteromeric complex: receptor I requires receptor II to bind TGF beta, and receptor II requires receptor I to signal. Mutations in T?R-I and T?R-II gene have been associated with Loeys-Dietz aortic aneurysm syndrome (LDAS).

NCBI and Uniprot Product Information

• NCBI GI #: 77695935
• NCBI GeneID: 29591
• NCBI Accession #: NP_036907.2
• NCBI GenBank Nucleotide #: NM_012775.2
• UniProt Accession #: P80204
• Molecular Weight: ~55 kDa
• NCBI Official Full Name: TGF-beta receptor type-1
• NCBI Official Synonym Full Names: transforming growth factor, beta receptor 1
• NCBI Official Symbol: Tgfbr1
• NCBI Official Synonym Symbols: Alk5; Skr4; Tgfr-1; tbetaR-I
• NCBI Protein Information: TGF-beta receptor type-1
• UniProt Protein Name: TGF-beta receptor type-1
• UniProt Gene Name: Tgfbr1
• UniProt Synonym Gene Names: TGFR-1; SKR4; TGF-beta receptor type I; TbetaR-I

NCBI Description

forms a heteromeric receptor complex with TGF-beta type II receptor that binds TGF-beta and mediates TGF-beta signalling [RGD, Feb 2006]

Uniprot Description

TGFBR1: a TKL kinase of the serine/threonine-protein kinase receptor (STKR) family. R1 and R2 TGF-beta receptors dimerize after binding TGF-beta at the cell surface. Found in all tissues; most abundant in placenta and least abundant in brain and heart.

Protein type: EC 2.7.11.30; Kinase, protein; Membrane protein, integral; Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; Receptor, misc.; STKR family; TKL group; Type1 subfamily

Chromosomal Location of Human Ortholog: 5q22

Cellular Component: apical plasma membrane; basolateral plasma membrane; caveola; cell; cell surface; endosome; intracellular membrane-bound organelle; lipid raft; membrane; plasma membrane; protein complex; receptor complex; tight junction

Molecular Function: ATP binding; growth factor binding; protein complex binding; protein heterodimerization activity; protein kinase activity; protein serine/threonine kinase activity; punt binding; receptor binding; receptor signaling protein activity; receptor signaling protein serine/threonine kinase activity; SMAD binding; transforming growth factor beta binding; transforming growth factor beta receptor activity; transforming growth factor beta receptor activity, type I; ubiquitin protein ligase binding

Biological Process: activation of MAPKK activity; activin receptor signaling pathway; aging; angiogenesis; anterior/posterior pattern formation; artery morphogenesis; blastocyst development; cell motility involved in cell locomotion; collagen fibril organization; embryo implantation; embryonic cranial skeleton morphogenesis; endothelial cell activation; endothelial cell migration; epithelial to mesenchymal transition; germ cell migration; gut development; heart development; in utero embryonic development; kidney development; lens development in camera-type eye; lung development; male gonad development; mesenchymal cell differentiation; negative regulation of apoptosis; negative regulation of chondrocyte differentiation; negative regulation of endothelial cell differentiation; negative regulation of endothelial cell proliferation; neuron fate commitment; organ regeneration; palate development; parathyroid gland development; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; pharyngeal system development; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell motility; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; positive regulation of filopodium formation; positive regulation of protein kinase B signaling cascade; positive regulation of stress fiber formation; positive regulation of transcription, DNA-dependent; post-embryonic development; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cardiac muscle cell proliferation; regulation of gene expression; regulation of protein binding; regulation of protein ubiquitination; regulation of transcription, DNA-dependent; response to electrical stimulus; response to estrogen stimulus; response to hypoxia; response to organic cyclic substance; response to toxin; signal transduction; skeletal development; skeletal morphogenesis; thymus development; transforming growth factor beta receptor signaling pathway