Western Blot (WB)
(Anti- Dicer antibody, Western blottingAll lanes: Anti Dicer at 0.5ug/mlWB: Recombinant Human Dicer Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)
Dicer Polyclonal Antibody | anti-DICER1 antibody
Anti-Dicer Antibody
Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml; tested Species: Human; Antigen Retrieval: By heat
WB: The detection limit for dicer is approximately 0.25ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0 for 20mins is required for the staining of formalin/paraffin sections.
Other application have not been tested.
Optimal dilutions should be determined by end userss.
Western Blot (WB)
(Anti- Dicer antibody, Western blottingAll lanes: Anti Dicer at 0.5ug/mlWB: Recombinant Human Dicer Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)
Western Blot (WB)
(Anti- Dicer antibody, Western blottingAll lanes: Anti Dicer at 0.5ug/mlWB: Recombinant Human Dicer Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)
Western Blot (WB)
(Anti- Dicer antibody, Western blottingAll lanes: Anti Dicer at 0.5ug/mlLane 1: Hela Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 217KDObserved bind size: 217KD)
Western Blot (WB)
(Anti- Dicer antibody, Western blottingAll lanes: Anti Dicer at 0.5ug/mlLane 1: Hela Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 217KDObserved bind size: 217KD)
Immunohistochemistry (IHC)
(Anti- Dicer antibody, IHC(P)IHC(P): Human Mammary Cancer Tissue)
Immunohistochemistry (IHC)
(Anti- Dicer antibody, IHC(P)IHC(P): Human Mammary Cancer Tissue)
Background: Dicer(DICER1), also known as endoribonuclease Dicer or helicase with RNase motif, is an enzyme that in humans is encoded by the DICER1 gene. It is mapped to 14q32.13. The DICER1 gene, a member of the ribonuclease III (RNaseIII) family, is involved in the generation of microRNAs (miRNAs), which modulate gene expression at the posttranscriptional level. DICER1 possesses an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus DICER, also known as helicase-MOI, is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. In addition, DICER1 is required for formation of the RNA induced silencing complex (RISC). It also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]
Uniprot Description
DICER1: a cytoplasmic type-III RNase that plays a central role in RNA interference (RNAi) pathways to produce the active small RNAs that represses gene expression. Possesses a DEAD box RNA helicase motif in its amino terminus and a dsRNA-binding motif in the carboxy terminus. Dicer, along with Ago2 andTRBP, are the main components of the RNA-induced silencing complex (RISC). Fragile X syndrome repeats form RNA hairpins that are cut by Dicer but do not activate the interferon-inducible protein kinase, PKR. Four alternatively spliced isoforms have been described.
Protein type: Ribonuclease; EC 3.1.26.3; RNA-binding; Helicase; RNA processing; Cell development/differentiation
Chromosomal Location of Human Ortholog: 14q32.13
Cellular Component: axon; cytoplasm; cytosol; dendrite; ER-Golgi intermediate compartment; growth cone; nucleus
Molecular Function: ATP binding; deoxyribonuclease I activity; double-stranded RNA binding; endoribonuclease activity; helicase activity; metal ion binding; protein binding; protein domain specific binding; ribonuclease III activity; siRNA binding
Biological Process: DNA fragmentation during apoptosis; miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, production of miRNAs; myelin formation in the peripheral nervous system; negative regulation of transcription from RNA polymerase II promoter; nerve development; neurite morphogenesis; positive regulation of myelination; positive regulation of Schwann cell differentiation; pre-microRNA processing; RNA interference, conversion of ds siRNA to ss siRNA; RNA interference, production of siRNA; RNA interference, siRNA loading onto RISC; RNA interference, targeting of mRNA for destruction; RNA-mediated gene silencing
Disease: Goiter, Multinodular 1, With Or Without Sertoli-leydig Cell Tumors; Pleuropulmonary Blastoma; Rhabdomyosarcoma, Embryonal, 2
Research Articles on DICER1
Similar Products
Product Notes
The DICER1 dicer1 (Catalog #AAA178064) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Dicer can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot Concentration: 0.1-0.5ug/ml; Tested Species: Human Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml; tested Species: Human; Antigen Retrieval: By heat WB: The detection limit for dicer is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0 for 20mins is required for the staining of formalin/paraffin sections. Other application have not been tested. Optimal dilutions should be determined by end userss. Researchers should empirically determine the suitability of the DICER1 dicer1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dicer, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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