Rabbit TGFBR1 Polyclonal Antibody | anti-TGFBR1 antibody

Rabbit anti TGFBR1 (Phospho) Polyclonal Antibody

Cat. Num. AAA460549

• Gene Names: TGFBR1; AAT5; ALK5; ESS1; LDS1; MSSE; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4; tbetaR-I
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
• Synonyms: TGFBR1; Polyclonal Antibody; Rabbit anti TGFBR1 (Phospho) Polyclonal Antibody; Transforming Growth Factor beta Receptor Type 1 (TGFBR1); ALK5; anti-TGFBR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
• Form/Format: This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer
• Sequence Length: 507

• Applicable Applications for anti-TGFBR1 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Western Blot: 0.1-1 ug/ml; ELISA: 0.01-0.1 ug/ml; Immunoprecipitation: 2-5 ug/ml; IHC: 5-10 ug/ml; Flow cytometry: Not tested ; Optimal dilutions should be determined by researchers for the specific applications.
• Antigen Preparation: A synthetic peptide derived from internal sequence (-TS*GS*GS*G-) with a phosphorylation sites at Serine 187, 189 and 191 of mouse TGFbeta receptor type-1. This sequence is also identical within human, rat, mouse, chicken and other species.
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Western Blot

(The cell lysate derived from TGF beta peptide (10 ng/ml for 30 minutes) stimulated MCF-7 cell lysate was immunoprobed by Rabbit anti-TGFbetaR1 (Cat# MBS460549) at 1:1000. A major immunoreactive band is observed around ~55 kDa)

Related Product Information for anti-TGFBR1 antibody

Transforming growth factor ? isoforms (TGF-?1, 2, 3) play vital roles in regulating cellular growth and differentiation, and they signal through a highly restricted subset of receptors known as TGF-? type I receptor (T?R-I) and TGF-? type II receptor (T?R-II). Expression of T beta R-I is more ubiquitous than that of T beta R-II. There was no staining of T beta R-II in the central nervous system, while intense T beta R-I staining was found specifically in nervous tissues. Expression of T beta R-I and T beta R-II was mostly coincident with that of their ligands, suggesting that TGF-beta s act as multiple mediators during organogenesis. TGF-? binds with high affinity to its receptor, a transmembrane protein with a cytoplasmic serine/threonine kinase domain, to form a dynamic dimer with phosphorylation in the cytoplasmic Ser/Thr residuals which cause its kinase activity and association with another TGF beta-binding protein, the type I receptor, to signal growth inhibition and early gene responses.

NCBI and Uniprot Product Information

• NCBI GI #: 817050443
• NCBI GeneID: 7046
• NCBI Accession #: NP_001293139.1
• NCBI GenBank Nucleotide #: NM_001306210.1
• UniProt Accession #: P36897; Q6IR47; Q706C0; Q706C1
• Molecular Weight: ~55 kDa
• NCBI Official Full Name: TGF-beta receptor type-1 isoform 3
• NCBI Official Synonym Full Names: transforming growth factor beta receptor 1
• NCBI Official Symbol: TGFBR1
• NCBI Official Synonym Symbols: AAT5; ALK5; ESS1; LDS1; MSSE; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4; tbetaR-I
• NCBI Protein Information: TGF-beta receptor type-1
• UniProt Protein Name: TGF-beta receptor type-1
• Protein Family: TGF-beta receptor
• UniProt Gene Name: TGFBR1
• UniProt Synonym Gene Names: ALK5; SKR4; TGFR-1; ALK-5; ALK5; SKR4; TGF-beta receptor type I; TbetaR-I

NCBI Description

The protein encoded by this gene forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF-beta signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys-Dietz aortic aneurysm syndrome (LDAS). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

Transmembrane serine/threonine kinase forming with the TGF-beta type II serine/threonine kinase receptor, TGFBR2, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. For instance, TGFBR1 induces TRAF6 autoubiquitination which in turn results in MAP3K7 ubiquitination and activation to trigger apoptosis. Also regulates epithelial to mesenchymal transition through a SMAD-independent signaling pathway through PARD6A phosphorylation and activation.